Benign E. coli adhesion and biofilm formation on mannoside surfaces

甘露糖苷表面上的良性大肠杆菌粘附和生物膜形成

• 批准号: 1207583
• 负责人: Chengzhi Cai
• 金额: $ 39万
• 依托单位: University of Houston
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1207583&HistoricalAwards=false
• 关键词: Benign; E.; coli; adhesion; biofilm

ID: MPS/DMR/BMAT(7623) 1207583 PI: Cai, Chengzhi ORG: University of HoustonTitle: Benign E. coli adhesion and biofilm formation on mannoside surfacesINTELLECTUAL MERIT: The proposed research is relevant to a new strategy to prevent pathogen colonization and biofilm formation. This new strategy uses benign bacteria to form a live, protective biofilm on materials surfaces to fence off pathogens. The overall objective of the proposed research is to investigate systematically the role of surface presentation of mannoside ligands on the formation of biofilms of a benign E. coli strain expressing mannoside-binding type 1 fimbriae. The specific objectives are: (1) To optimize the surface presentation of mannoside ligands. A series of mannoside ligands will be synthesized, varying the substituents on the glycosidic linkages. They will be covalently attached to silicone surfaces via an intermittent layer of poly(amido amine) (PAMAM) dendrimers. This approach will allow for control of the local ligand density at nanoscale. (2) To address the role of the affinity and nanoscale presentation of the mannoside ligands on the adhesion, orientation, mobility, proliferation, extracellular matrix production, and aggregation of the benign E. coli, as well as the rate of formation, morphology, coverage, and stability of the benign biofilms. Great progress has been made in molecular biological study of the regulatory mechanism in the late stage of biofilm formation. In addition, the knowledge base has been growing rapidly on how the early stage of biofilm formation is affected by material surface physical, chemical, and mechanical properties. However, the understanding of the effect of specific ligand-receptor interactions on biofilm formation on materials surfaces is limited. The proposed research aims to fill this critical gap in knowledge. BROADER IMPACTS: Bacterial colonization and biofilm formation has a tremendous impact on human health and the environment. The proposed research will provide new insights to the process of biofilm formation, which will be invaluable for the development of strategies to prevent pathogen colonization and to promote the formation of benign or functional biofilms. Undergraduate and graduate students involved in the project will be trained in many fields. The PI will organize a workshop for the Science Extravaganza at the University of Houston to motivate high school students from underrepresented groups to choose a STEM (science, technology, engineering, mathematics) field major in college. An undergraduate student in this project will be recruited from the teach Houston program at the University of Houston. Students in the program teach classroom lessons in local high schools from their first year. The PI will work with the student to develop exciting modules, such as observation of benign biofilm formation, for use in the science course.

PI：蔡成志ORG: University of休斯顿标题：甘露糖苷表面的良性大肠杆菌粘附和生物膜形成智力优势：拟议的研究与预防病原体定植和生物膜形成的新策略有关。这种新策略利用良性细菌在材料表面形成一层活的、保护性的生物膜来隔离病原体。本研究的总体目标是系统地研究甘露糖苷配体的表面呈现对表达甘露糖苷结合型1型菌膜的良性大肠杆菌菌株生物膜形成的作用。具体目的是：(1)优化甘露糖苷配体的表面呈现。将合成一系列甘露糖苷配体，改变糖苷键上的取代基。它们将通过聚胺（PAMAM）树状大分子的间歇层共价附着在硅酮表面。这种方法将允许在纳米尺度上控制局部配体密度。(2)研究甘露糖苷配体的亲和性和纳米级表现对良性大肠杆菌的粘附、取向、迁移、增殖、细胞外基质的产生和聚集的影响，以及良性生物膜的形成速度、形态、覆盖范围和稳定性的影响。生物膜形成后期调控机制的分子生物学研究取得了很大进展。此外，关于生物膜形成的早期阶段如何受到材料表面物理、化学和机械性能的影响的知识库也在迅速增长。然而，对特定的配体-受体相互作用对材料表面生物膜形成的影响的理解是有限的。拟议的研究旨在填补这一关键的知识空白。广泛的影响：细菌定植和生物膜的形成对人类健康和环境有巨大的影响。本研究将为生物膜的形成过程提供新的见解，这对于制定防止病原体定植和促进良性或功能性生物膜的形成的策略具有宝贵的价值。参与该项目的本科生和研究生将接受多个领域的培训。PI将为休斯顿大学的科学盛会组织一个研讨会，以激励来自弱势群体的高中生在大学里选择STEM（科学、技术、工程、数学）领域的专业。本项目将从休斯顿大学的teach Houston项目中招募一名本科生。这个项目的学生从一年级开始就在当地高中教授课堂课程。PI将与学生一起开发令人兴奋的模块，例如观察良性生物膜的形成，用于科学课程。