DISSERTATION RESEARCH: Phylogeographic discord in the comparative genomic history of Amazonian birds

论文研究：亚马逊鸟类比较基因组历史中的系统地理学不一致

• 批准号: 1210556
• 负责人: Robb Brumfield
• 金额: $ 1.5万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1210556&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Phylogeographic; discord; comparative

The production of species diversity is of fundamental interest, but the factors that cause diversification are poorly understood. Diversification is known to occur at different rates depending on the species or group of species involved, and comparative study of this variation can reveal the processes that promote diversity. Comparative phylogeography is the study of diversification at the population level within different species, and it allows us to observe differences in diversification at the point where speciation occurs. The goals of this study are to use comparative phylogeography and novel methods of generating data to detect differences in diversification across species, and to analyze the factors that promote faster diversification in some species than in others. Phylogeography will be compared across sixteen Amazonian bird species with similar distributions. A new set of genetic markers scattered across the genome will be used to generate data from 24 individuals of each species. These genetic sequences will be extracted from museum tissue samples using a new method of enriching genomic DNA for sequences of interest, and then sequenced on a next-generation sequencing instrument. New analytical tools for testing models will allow us to measure the processes that are most important in driving the diversification of a given species. The massive amount of data generated by this method will allow for analysis of more complex models than could ever be analyzed previously, giving us new insight into the processes promoting species diversity. This project provides research opportunities for undergraduate students in Louisiana and the geographic scope of the project fosters inter-institutional and international collaborations. The research provides a test of next-generation sequencing methods and a new genomic marker set, and our results will aid in the application of these methods to other study systems. New biogeographic and geographic genetic data from our work support conservation efforts in the Amazon. All specimens, audio recordings, and observational data from our fieldwork and genetic data from our lab work are deposited in publicly accessible collection and databases. We consider these resources invaluable for current and future, pure and applied research on Amazonian birds and other systems.

物种多样性的产生具有根本意义，但对导致多样化的因素知之甚少。众所周知，多样化发生的速度不同，取决于所涉物种或物种组，对这种变化的比较研究可以揭示促进多样性的过程。比较物种地理学是研究不同物种在种群水平上的多样性，它使我们能够观察物种形成时多样性的差异。本研究的目标是使用比较地理学和新的数据生成方法来检测不同物种的多样化差异，并分析促进某些物种比其他物种更快多样化的因素。系统地理学将在16种分布相似的亚马逊鸟类之间进行比较。一组新的分散在基因组中的遗传标记将用于生成每个物种24个个体的数据。这些基因序列将使用一种新的方法从博物馆组织样本中提取，该方法富集基因组DNA中的感兴趣序列，然后在下一代测序仪器上进行测序。用于测试模型的新分析工具将使我们能够衡量在推动特定物种多样化方面最重要的过程。这种方法产生的大量数据将允许分析比以前分析的更复杂的模型，使我们对促进物种多样性的过程有了新的认识。该项目为路易斯安那州的本科生提供了研究机会，该项目的地理范围促进了机构间和国际合作。该研究提供了下一代测序方法和新的基因组标记集的测试，我们的结果将有助于将这些方法应用于其他研究系统。我们工作中获得的新的地理和地理遗传数据支持了亚马逊的保护工作。所有的标本，录音，从我们的实地工作和遗传数据从我们的实验室工作的观察数据存放在公开访问的集合和数据库。我们认为这些资源对当前和未来的亚马逊鸟类和其他系统的纯研究和应用研究非常宝贵。