An Automated Capillary Sequencer for Systematics, Population Genetics, Genomics, and Molecular Ecology

该奖项为获得现代毛细管DNA测序仪器提供了支持，该仪器能够每天以98.5%的准确率确定近200,000个碱基，并且还能够进行高通量片段分析，包括微卫星和单核苷酸多态性。该仪器将用于许多正在进行的项目，包括nsf资助的生命之树测序项目，该项目旨在产生所有鸟类的分子系统发育。仪器将被使用。除了在研究中发挥作用外，该仪器还将用于高中、本科、研究生和博士后水平的培训。该仪器将取代现有的平板-凝胶仪器，该仪器已被大量用于研究鸟类系统的种群遗传学和进化基因组学，哺乳动物及其寄生虱子的共同进化遗传学，以及爬行动物和两栖动物的进化遗传学。新仪器将提供数倍的测序能力，可用于自然历史博物馆的这些和其他项目。

This award provides support for acquisition of a modern capillary DNA sequencing instrument capable of determining nearly 200,000 bases a day at 98.5% accuracy, and also capable of high throughput fragment analysis including that of microsatellites and single nucleotide polymorphisms. The instrument will be used in a number of ongoing projects, including an NSF-funded Tree of Life sequencing project aimed at producing a molecular phylogeny of all birds. The instrument will be used. In addition to its role in research, the instrument will be used for training at the high school, undergraduate, graduate, and post-doctoral levels. The instrument will replace an existing slab-gel instrument that has been heavily used for studies of population genetics and evolutionary genomics of avian systems, co-evolutionary genetics of mammals and their parasitic lice, and evolutionary genetics of reptiles and amphibians. The new instrument will provide a several-fold increase in the sequencing capacity that can be brought to bear on these and other projects at the Museum of Natural History.