DISSERTATION RESEARCH: Diversification and species delimitation in lizard malaria parasites

论文研究：蜥蜴疟原虫的多样化和物种界定

• 批准号: 1210547
• 负责人: Susan Perkins
• 金额: $ 1.45万
• 依托单位: American Museum Natural History
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1210547&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Diversification; species; delimitation

Malaria parasites in the genus Plasmodium are well known as causing disease in humans, but half of the roughly 200 species in this genus use lizards as their vertebrate hosts, and many of these species can be found in lizards in Panama. These parasites have been the focus of studies on ecology, epidemiology, and behavior, but their taxonomic classifications and patterns of diversification remain unclear, and this has hindered their use as a model for studying disease. The first goal of this study is to resolve the taxonomy of these Panamanian parasites. Malaria parasite species are traditionally classified based on differences in their morphology, but the simple and changing morphology of the Panamanian species has confounded taxonomists for decades. Their taxonomy will be revised by analyzing genetic information using novel species-delimitation methods. The second goal of this study is to clarify the patterns of diversification in the widespread lizard parasite Plasmodium floridense. It is distributed in southern North America, throughout the Caribbean, and in Middle America, but like most malaria parasites, its history of colonization remains unknown. Newly collected samples from Panama will be combined with previously collected samples from across the parasite?s range, and these will be used to elucidate the parasite?s pattern of diversification. The results of this research will be disseminated broadly and used to promote teaching and learning. Curriculum materials will be developed in partnership with the Gottesman Center at the American Museum of Natural History (AMNH) to be used in New York City high school biology classrooms. Lectures and laboratory activities will also be developed as part of the Science Research Mentoring Program at the AMNH. Both will introduce urban high school students to the vast diversity of parasite species from across the tree of life. Finally, results of this research will be posted over several entries on two blogs, each of which is well established and has a wide readership, to facilitate the dissemination of the research to the general public.

众所周知，疟原虫属的疟疾寄生虫会引起人类疾病，但该属的大约200种中有一半使用蜥蜴作为其脊椎动物宿主，其中许多物种可以在巴拿马的蜥蜴中找到。 这些寄生虫一直是生态学、流行病学和行为学研究的焦点，但它们的分类和多样化模式仍不清楚，这阻碍了它们作为疾病研究模型的使用。 本研究的第一个目标是解决这些巴拿马寄生虫的分类。 疟疾寄生虫物种传统上是根据其形态的差异进行分类的，但巴拿马物种的简单和不断变化的形态几十年来一直困扰着分类学家。 它们的分类学将通过使用新的物种界定方法分析遗传信息来修订。 本研究的第二个目标是澄清在广泛分布的蜥蜴寄生虫Plasmodium floridense多样化的模式。 它分布在北美洲南部，整个加勒比海和中美洲，但像大多数疟疾寄生虫一样，其殖民历史仍然未知。 从巴拿马新收集的样本将与以前从整个寄生虫收集的样本相结合？的范围，这些将被用来阐明寄生虫？的多样化模式。 这项研究的结果将广泛传播，并用于促进教学和学习。 课程材料将与美国自然历史博物馆（AMNH）的Gottesman中心合作开发，用于纽约市高中生物课堂。 讲座和实验室活动也将作为AMNH科学研究指导计划的一部分。 两者都将向城市高中学生介绍来自生命之树的寄生虫物种的巨大多样性。 最后，这项研究的结果将在两个博客的几个条目上公布，每个博客都很成熟，读者群很广，以促进向公众传播研究结果。