Karyotype and genome evolution in Brassicaceae Lineage III. Comparative karyotypic studies through multicolour chromosome painting

比较染色体涂色（CCP）能够识别密切相关的类群之间共享的大同源染色体区域。在植物中，该技术迄今为止仅适用于十字花科植物（十字花科）。拟议的项目将阐明十字花科谱系 III 中的染色体重排，并旨在确定该谱系的祖先核型。这将通过确定谱系 III 中所有七个部落代表的染色体上祖先基因组块（在之前的研究中定义）的位置来实现。 CCP 还将用于揭示谱系 III 内有限物种组内属下和种内核型变异的程度。结果将根据十字花科祖先核型（ACK）以及十字花科谱系 I 和 II 的现有结果进行分析，并放入系统发育框架中。这将扩展我们对十字花科和植物界内跨物种染色体共线性程度、核型改变情景和速率的了解。

Comparative chromosome painting (CCP) enables the identification of large homeologous chromosome regions shared among closely related taxa. In plants, the technique so far is only feasible in crucifers (Brassicaceae). The proposed project will elucidate chromosome rearrangements in the Brassicaceae Lineage III and aims to identify the ancestral karyotype for that lineage. This will be achieved through identifying the position of the ancestral genomic blocks (defined in previous studies) on the chromosomes of representatives of all seven tribes within Lineage III. CCP will also be applied to unveil the extent of infrageneric and intraspecific karyotypic variation within a confined species group within lineage III. The results will be analysed in relation to the Ancestral Crucifer Karyotype (ACK) as well as existing results of Brassicaceae Lineages I and II and also put into a phylogenetic framework. This will expand our knowledge on the extent of cross-species chromosomal colinearity, scenarios and rates of karyotypic alterations within Brassicaceae and the plant kingdom.