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Dynamics of Large RNAs Studied Using Site-Directed Spin Labeling

使用定点自旋标记研究大 RNA 的动力学

基本信息

批准号：
1213673
负责人：
Peter Qin
金额：
$ 33万
依托单位：
University of Southern California
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-15 至 2017-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1213673&HistoricalAwards=false
关键词：
Dynamics Large RNAs Studied Using

项目摘要

In this award from the Chemistry of Life Processes Program in the Division of Chemistry, Dr. Peter Qin, from the University of Southern California, will investigate intrinsic dynamics in the nano- to microsecond regime of a large ribozyme using site-directed spin labeling. By monitoring nitroxide spin labels covalently attached to specific RNA sites with electron paramagnetic resonance spectroscopy, information on RNA dynamics will be obtained at various equilibrium states of wild type and mutant ribozymes. The studies aim to: (i) reveal sequence and structure elements that dictate intrinsic RNA dynamics, and (ii) correlate intrinsic dynamics to RNA folding and catalysis. The goal of the project is to advance fundamental understanding of the structure-dynamics-function relationship in RNA.The broader impact of the proposed work stems from advancing experimental methods for probing biological systems, enhancing research infrastructure and resources, and providing knowledge that benefits the broader scientific community. The proposed work will contribute to student training at all levels, with emphasis on using a multidisciplinary approach and on promoting participation of members from under-represented groups (e.g. minority undergraduates from local primarily undergraduate institutions) in research. Examples from the Dr. Qin's research are incorporated into courses and education programs to propel the growth of the Chemistry Department and the University.

在化学系生命过程计划的化学奖中，南加州大学的Peter Qin博士将使用定点自旋标记研究大型核酶在纳米至微秒范围内的内在动力学。通过监测氮氧自旋标记共价连接到特定的RNA网站与电子顺磁共振光谱，RNA动力学的信息将获得在各种平衡状态的野生型和突变型核酶。这些研究旨在：（i）揭示决定内在RNA动力学的序列和结构元素，以及（ii）将内在动力学与RNA折叠和催化相关联。该项目的目标是推进对RNA中结构-动力学-功能关系的基本理解。拟议工作的更广泛影响源于推进探测生物系统的实验方法，增强研究基础设施和资源，并提供有利于更广泛科学界的知识。拟议的工作将有助于各级学生的培训，重点是采用多学科方法，并促进代表性不足群体的成员（例如，当地主要是本科院校的少数民族本科生）参与研究。秦博士的研究成果被纳入课程和教育计划，以推动化学系和大学的发展。