Directly quantifying the isotopic fractionation associated with the key enzymes in microbial sulfate reduction

直接量化与微生物硫酸盐还原中关键酶相关的同位素分级分离

基本信息

  • 批准号:
    1225980
  • 负责人:
  • 金额:
    $ 23.92万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2012
  • 资助国家:
    美国
  • 起止时间:
    2012-08-15 至 2015-07-31
  • 项目状态:
    已结题

项目摘要

The microbial lifestyle of sulfate reduction is central to the oxidation of organic matter both in the Modern and throughout Earth?s history. This linkage to the carbon cycle, and in turn climate, is commonly inferred through sulfur isotope studies, which are themselves informed by experiments with extant bacteria that perform this process. At its core, however, bacteria are driven by a suite of enzymes, or catalysts, that conduct much of the critical chemistry. In this proposal, investigators look to place the first direct isotopic constraints on the two key enzymatic steps within this metabolism: dissimilatory sulfite reductase [DsrAB/C] and APS oxido-reductase [APSr]. This data will allow for a more mechanistic understanding of cellular fractionation processes, which will then sharpen our capacity to read environmental records.This research project will be conducted in collaboration with Dr. Ines Pereira of the Instituto De Technologia Quimica E Biologica, ITQB, Portugal, who will provide the biochemistry expertise necessary for the project. A Harvard graduate student will travel between the Pereira and Johnston's labs, acquiring valuable interdisciplinary training in isotope geochemistry and biochemistry. Travel costs for the international component of this research have been provided by the NSF Office of International Science and Engineering.
硫酸盐还原的微生物生活方式是现代和整个地球有机物氧化的核心。年代的历史。这种与碳循环和气候的联系通常是通过硫同位素研究推断出来的,而硫同位素研究本身是通过对现存细菌进行这一过程的实验得出的。然而,在其核心,细菌是由一套酶或催化剂驱动的,这些酶或催化剂进行了许多关键的化学反应。在这项提议中,研究人员希望对这一代谢过程中的两个关键酶步骤:异化亚硫酸盐还原酶(DsrAB/C)和APS氧化还原酶(APSr)进行第一次直接的同位素限制。这些数据将允许对细胞分馏过程有更机械的理解,这将提高我们读取环境记录的能力。该研究项目将与葡萄牙ITQB Quimica E bi研究所的Ines Pereira博士合作进行,他将为该项目提供必要的生物化学专业知识。一名哈佛研究生将在佩雷拉和约翰斯顿的实验室之间穿梭,在同位素地球化学和生物化学方面获得宝贵的跨学科培训。本次研究国际部分的旅费由美国国家科学基金会国际科学与工程办公室提供。

项目成果

期刊论文数量(0)
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David Johnston其他文献

An Action Research on Exploring Disaster Reduction Activities Embedded in the Daily Life
探索减灾活动融入日常生活的行动研究
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Michael K. Lindell;Carla S. Prater;Hao Che Wu;Shih-Kai Huang;David Johnston;Julia Becker and Hideyuki Shiroshita;城下英行・原愛樹・玉置崇・澤平敏秀・斉藤早苗;城下英行;Hideyuki Shiroshita
  • 通讯作者:
    Hideyuki Shiroshita
Development of WebGIS System for Supporting New Disaster Education
支持新型灾害教育的WebGIS系统开发
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Michael K. Lindell;Carla S. Prater;Hao Che Wu;Shih-Kai Huang;David Johnston;Julia Becker and Hideyuki Shiroshita;城下英行・原愛樹・玉置崇・澤平敏秀・斉藤早苗;城下英行;Hideyuki Shiroshita;城下英行;Hideyuki Shiroshita;Hideyuki Shiroshita
  • 通讯作者:
    Hideyuki Shiroshita
学び合う防災学習の理論と実践
防灾理论与实践学习互学互鉴
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Michael K. Lindell;Carla S. Prater;Hao Che Wu;Shih-Kai Huang;David Johnston;Julia Becker and Hideyuki Shiroshita;城下英行・原愛樹・玉置崇・澤平敏秀・斉藤早苗;城下英行;Hideyuki Shiroshita;城下英行;Hideyuki Shiroshita;Hideyuki Shiroshita;城下英行
  • 通讯作者:
    城下英行
Multilayer Disaster Education through Collaboration between a Disaster Education Centre and a Local University
灾害教育中心与当地大学合作开展多层次灾害教育
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Michael K. Lindell;Carla S. Prater;Hao Che Wu;Shih-Kai Huang;David Johnston;Julia Becker and Hideyuki Shiroshita;城下英行・原愛樹・玉置崇・澤平敏秀・斉藤早苗;城下英行;Hideyuki Shiroshita;城下英行;Hideyuki Shiroshita;Hideyuki Shiroshita;城下英行;Hideyuki Shiroshita;城下英行・原愛樹・玉置崇・澤平敏秀・斉藤早苗;Hideyuki Shiroshita;Hideyuki Shiroshita
  • 通讯作者:
    Hideyuki Shiroshita
How Visual Design of Severe Weather Outlooks Can Affect Communication and Decision-Making
恶劣天气前景的视觉设计如何影响沟通和决策
  • DOI:
    10.1175/wcas-d-23-0010.1
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Mary Anne T. Clive;Emma E. H. Doyle;S. Potter;Chris Noble;David Johnston
  • 通讯作者:
    David Johnston

David Johnston的其他文献

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{{ truncateString('David Johnston', 18)}}的其他基金

Collaborative Research: EAGER--Novel Sampling and Isotopic Characterization of Upper Strato- to Mesospheric Photochemistry
合作研究:EAGER——上平层至中层光化学的新型采样和同位素表征
  • 批准号:
    2204475
  • 财政年份:
    2022
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
Clumped Oxygen Isotope Signature of Marine Dissolved Oxygen
海洋溶解氧的聚集氧同位素特征
  • 批准号:
    2049298
  • 财政年份:
    2021
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
Collaborative Research: Common Environmental Drivers Determine the Occupation Chronology of Adélie Penguins and Moss Peatbanks on the Western Antarctic Peninsula
合作研究:共同的环境驱动因素决定了南极半岛西部阿德利企鹅和苔藓泥炭滩的生活年表
  • 批准号:
    2012365
  • 财政年份:
    2021
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
Collaborative Research: Unlocking the Cenozoic/Cretaceous seawater sulfate record via inclusion of 17O in marine barite
合作研究:通过海洋重晶石中的 17O 来解锁新生代/白垩纪海水硫酸盐记录
  • 批准号:
    1946137
  • 财政年份:
    2020
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
Collaborative Research: Development of a novel way for understanding ancient Earth atmospheres and marine sulfate using the stable isotope of Oxygen (17O) in marine barite.
合作研究:利用海洋重晶石中氧(17O)的稳定同位素开发一种了解古代地球大气和海洋硫酸盐的新方法。
  • 批准号:
    1821958
  • 财政年份:
    2018
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
EAGER: Unraveling riverine sulfate using minor oxygen isotopes
EAGER:使用次要氧同位素解开河流硫酸盐
  • 批准号:
    1839341
  • 财政年份:
    2018
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
A marine Unoccupied Aircraft Systems (UAS) facility: Building capacity for high-resolution remote sensing and analysis of marine and coastal ecosystems
海洋无人飞机系统(UAS)设施:建设高分辨率遥感以及海洋和沿海生态系统分析的能力
  • 批准号:
    1624645
  • 财政年份:
    2016
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
Collaborative Research: Experimental calibration of the isotopic content of marine sulfate
合作研究:海洋硫酸盐同位素含量的实验校准
  • 批准号:
    1536574
  • 财政年份:
    2015
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Standard Grant
Collaborative Research: Oxygen, Ecology and the Cambrian radiation of Animals
合作研究:动物的氧气、生态和寒武纪辐射
  • 批准号:
    1325128
  • 财政年份:
    2013
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Continuing Grant
CAREER: Quantifying the flux and isotope effects associated with sulfur-bearing intermediate species within dissimilatory sulfate reduction
职业:量化与异化硫酸盐还原中含硫中间物质相关的通量和同位素效应
  • 批准号:
    1149555
  • 财政年份:
    2012
  • 资助金额:
    $ 23.92万
  • 项目类别:
    Continuing Grant

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量化气候变化对农业景观中湿地的影响
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