Minority Starter Grant - Development of Microsatellite Markers for Cranberry Genetic Improvement

少数启动补助金 - 蔓越莓遗传改良微卫星标记的开发

• 批准号: 1228280
• 负责人: Juan Zalapa
• 金额: $ 5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1228280&HistoricalAwards=false
• 关键词: Minority; Starter; Grant; Development; Microsatellite

The cranberry (Vaccinium macrocarpon Ait.) is a commercially important, North American, woody plant species in the Ericaceae family. Next-generation sequencing technologies offer the possibility of generating large numbers of quality molecular markers quickly and cheaply. The use of such markers will allow the development of innovative plant breeding systems to speed the breeding of unique cranberry cultivars. This research will (1) use Roche 454 pyrosequencing data for the development and testing of 2,400 primer pairs, (2) develop 250 to 500 microsatellite polymorphic markers for cultivated and natural germplasm characterization and linkage map construction, and (3) develop a microsatellite marker based fingerprinting assay for the true-to-type verification of clonally propagated cranberry cultivars. After development and testing, the resulting microsatellite markers will be particularly useful in cranberry genetic studies due to high reproducibility, their multiallelic nature, codominance, relative abundance, wide dispersal in most plant genomes, and transferability among closely related species.The cranberry is a commercially important, North American, woody plant species. The U.S. is a worldwide leading producer of cranberries, which are highly prized for their tart flavor and nutritional and medicinal attributes. However, little information is available regarding the genetics of the species. Next-generation sequencing technologies offer the possibility of generating large numbers of quality molecular markers quickly and cheaply. The use of such markers will allow the development of innovative plant breeding systems to speed the breeding of unique cranberry cultivars to meet challenges such as increasing yield in sustainable production systems, improving berry quality (including nutrition), and responding to increasingly variable and extreme climates and insects and diseases pressures. This project will help support an ongoing education biotech outreach program targeting diverse audiences.

蔓越橘（Vaccinium macrocarpon Ait.）是杜鹃花科的一种重要的北美木本植物。下一代测序技术提供了快速和廉价地产生大量优质分子标记的可能性。这些标记的使用将允许开发创新的植物育种系统，以加速独特的蔓越莓品种的育种。本研究将（1）使用罗氏454焦磷酸测序数据开发和测试2,400对引物，（2）开发250至500个微卫星多态性标记用于栽培和天然种质表征和连锁图谱构建，（3）开发基于微卫星标记的指纹分析用于克隆繁殖蔓越莓品种的真实类型验证。经过开发和测试，所产生的微卫星标记将是特别有用的蔓越莓遗传学研究，由于高重复性，多等位基因的性质，共显性，相对丰度，广泛分布在大多数植物基因组中，和近缘物种之间的可转移性。蔓越莓是一种重要的商业，北美，木本植物物种。美国是蔓越莓的全球领先生产国，蔓越莓因其酸味、营养和药用特性而备受推崇。然而，关于该物种的遗传学信息很少。下一代测序技术提供了快速和廉价地产生大量优质分子标记的可能性。这些标记的使用将允许开发创新的植物育种系统，以加速独特的蔓越莓品种的育种，以应对挑战，例如在可持续生产系统中提高产量，改善浆果质量（包括营养），并应对日益多变和极端的气候以及昆虫和疾病的压力。该项目将帮助支持一个针对不同受众的持续教育生物技术推广计划。