SBIR Phase II: Correlation of Surface Free Energy and Cytocompatibility of Amphiphilic Biomaterials

该小型企业创新研究（SBIR）第二阶段项目涉及使用新型两亲性聚合物在无刺痛性、挥发性疏水性溶剂中的喷涂聚合物涂层的开发，用于治疗一度和二度烧伤伤口，减少瘢痕形成，其中形成用于人细胞附着、活力和增殖的透明、柔性基底，而不添加任何外源性生物组分。目前不存在治疗烧伤的方法，其在治疗时同时减轻疼痛，与规则和不规则（面部、手指）伤口表面紧密共形，提供用于伤口闭合和愈合的细胞基质，并且不需要去除敷料，例如使用所提出的喷涂聚合物涂层。这项调查将涉及这些新型聚合物的细胞相容性和伤口愈合的研究，结合聚合物的测定？喷涂特性所需的物理和机械性能。这项研究的成功将证明，一个复杂的，但便宜，易于应用，保形聚合物涂层可用于烧伤治疗，减少疤痕。该项目更广泛的影响/商业潜力涉及一种新型喷涂透明聚合物涂层的商业化，该涂层由无刺痛感、挥发性疏水性溶剂提供，用于治疗一度和二度烧伤，减少疤痕形成。烧伤是医疗保健中最昂贵的方面之一。全世界每年有超过4000万疤痕患者，治疗费用为120亿美元。目前，没有商业烧伤护理产品或方法利用诸如本研究给出的患者和烧伤者友好的技术来提供改善的烧伤愈合，同时减少疼痛和减少疤痕形成。尽管在用于制备局部伤口护理的支架的组织工程中进行了大量研究，但少数商业化产品在制备、储存和使用方面成本高，并且易于降解和丧失活性。随着该项目的成功，将开发一种新技术，该技术显著改善了烧伤治疗，该技术基于在市售溶剂中容易合成的稳定聚合物，该技术提供了一种透明的、水不溶性的、氧气和水蒸气可渗透的涂层，适用于大面积烧伤伤口，而不形成拼凑设计，并且随着伤口愈合而自行去除。

This Small Business Innovation Research (SBIR) Phase II project pertains to the development of a spray-on polymer coating using a novel amphiphilic polymer in a non-stinging, volatile hydrophobic solvent for treatment of first and second degree burn wounds with reduction of scar formation, wherein a transparent, flexible substrate for human cell attachment, viability and proliferation is formed without any added exogenous biological components. No methods currently exist for treating burns that concurrently reduce pain upon treatment, are intimately conformal to regular and irregular (face, fingers) wound surfaces, provide a cell substrate for wound closure and healing, and do not require dressing removal, such as with the proposed spray-on polymer coating. This investigation will involve cytocompatibility and wound healing studies of these novel polymers, in conjunction with a determination of the polymers? physical and mechanical properties needed for spray-on characteristics. The success of this investigation will demonstrate that a sophisticated, yet inexpensive, easily applied, conformal polymer coating can be used for burn treatment with reduction of scarring. The broader impact/commercial potential of this project pertains to the commercialization of a novel spray-on transparent polymer coating, delivered from a non-stinging, volatile hydrophobic solvent, to treat first and second degree burn injuries, with reduction of scar formation. Burn injuries constitute one of the most expensive aspects of health care. There are more than 40 million scar patients worldwide per year, at a treatment cost of $12 billion. Currently, no commercial burn care product or methodology provides for improved burn healing with reduced pain and reduced scar formation utilizing a patient and caregiver-friendly technique such as given by this investigation. Despite substantial research in tissue engineering for the preparation of scaffolds for topical wound care, the few commercialized products are costly in preparation, storage and use, and are subject to degradation and loss of activity. With the success of this project, a new technology will be developed that significantly improves burn treatment, is based upon readily synthesized, stable polymers in a commercially-available solvent, that provides a transparent, water-insoluble, oxygen- and water-vapor permeable coating suitable for use over large area burn wounds without forming a patch-work design, and which self-removes as the wound heals.