SBIR Phase II: Correlation of Surface Free Energy and Cytocompatibility of Amphiphilic Biomaterials

这个小型企业创新研究(SBIR)第二阶段项目涉及开发一种喷雾聚合物涂层，该涂层使用一种新型的两亲性聚合物，在无刺痛的挥发性疏水溶剂中用于治疗一度和二度烧伤创面，减少疤痕形成，其中形成了透明、灵活的基质，用于人体细胞附着、生存和增殖，而不需要添加任何外源生物成分。目前还没有治疗烧伤的方法，这些方法可以同时减轻治疗时的疼痛，与规则和不规则(面部、手指)创面紧密共形，为伤口闭合和愈合提供细胞基质，并且不需要去除敷料，例如使用所提议的喷涂聚合物涂层。这项研究将包括这些新型聚合物的细胞相容性和伤口愈合研究，以及聚合物的测定？喷涂特性所需的物理和机械性能。这项研究的成功将证明，一种复杂、廉价、易于应用的共形聚合物涂层可以用于烧伤治疗，减少瘢痕形成。该项目的更广泛的影响/商业潜力与一种新型的喷涂透明聚合物涂层的商业化有关，该涂层由一种无刺痛的挥发性疏水溶剂提供，用于治疗一度和二度烧伤，减少疤痕形成。烧伤是医疗保健最昂贵的方面之一。全世界每年有4000多万疤痕患者，治疗费用为120亿美元。目前，还没有商业化的烧伤护理产品或方法能够利用患者和护理者友好的技术来改善烧伤愈合，同时减少疼痛和疤痕形成，例如本研究给出的技术。尽管在组织工程学方面进行了大量研究，以准备用于局部伤口护理的支架，但为数不多的商业化产品在准备、储存和使用方面成本高昂，并且容易降解和失去活性。随着该项目的成功，将开发一种显著改善烧伤治疗的新技术，该技术基于在商业可获得的溶剂中容易合成的稳定聚合物，提供一种透明、不溶于水、透氧和透水蒸汽的涂层，适用于大面积烧伤创面，而不形成补丁设计，并且随着伤口的愈合自行去除。

This Small Business Innovation Research (SBIR) Phase II project pertains to the development of a spray-on polymer coating using a novel amphiphilic polymer in a non-stinging, volatile hydrophobic solvent for treatment of first and second degree burn wounds with reduction of scar formation, wherein a transparent, flexible substrate for human cell attachment, viability and proliferation is formed without any added exogenous biological components. No methods currently exist for treating burns that concurrently reduce pain upon treatment, are intimately conformal to regular and irregular (face, fingers) wound surfaces, provide a cell substrate for wound closure and healing, and do not require dressing removal, such as with the proposed spray-on polymer coating. This investigation will involve cytocompatibility and wound healing studies of these novel polymers, in conjunction with a determination of the polymers? physical and mechanical properties needed for spray-on characteristics. The success of this investigation will demonstrate that a sophisticated, yet inexpensive, easily applied, conformal polymer coating can be used for burn treatment with reduction of scarring. The broader impact/commercial potential of this project pertains to the commercialization of a novel spray-on transparent polymer coating, delivered from a non-stinging, volatile hydrophobic solvent, to treat first and second degree burn injuries, with reduction of scar formation. Burn injuries constitute one of the most expensive aspects of health care. There are more than 40 million scar patients worldwide per year, at a treatment cost of $12 billion. Currently, no commercial burn care product or methodology provides for improved burn healing with reduced pain and reduced scar formation utilizing a patient and caregiver-friendly technique such as given by this investigation. Despite substantial research in tissue engineering for the preparation of scaffolds for topical wound care, the few commercialized products are costly in preparation, storage and use, and are subject to degradation and loss of activity. With the success of this project, a new technology will be developed that significantly improves burn treatment, is based upon readily synthesized, stable polymers in a commercially-available solvent, that provides a transparent, water-insoluble, oxygen- and water-vapor permeable coating suitable for use over large area burn wounds without forming a patch-work design, and which self-removes as the wound heals.