SBIR Phase II: Correlation of Surface Free Energy and Cytocompatibility of Amphiphilic Biomaterials

这个小型企业创新研究（SBIR）二期项目涉及一种喷涂聚合物涂层的开发，该涂层使用一种新型的两亲性聚合物，在一种无刺痛、易挥发的疏水溶剂中，用于治疗一、二度烧伤伤口，减少疤痕形成，其中透明、柔性的基底用于人体细胞附着、活力和增殖，不需要任何外源性生物成分。目前还没有一种治疗烧伤的方法可以同时减轻治疗时的疼痛，与规则和不规则（面部、手指）伤口表面保持密切的适形，为伤口闭合和愈合提供细胞基质，并且不需要去除敷料，例如使用拟议的喷涂聚合物涂层。本研究将涉及这些新型聚合物的细胞相容性和伤口愈合研究，并结合聚合物的测定？喷涂特性所需的物理和机械性能。这项研究的成功将证明，一种复杂、廉价、易于应用的保形聚合物涂层可以用于减少疤痕的烧伤治疗。该项目的更广泛影响/商业潜力涉及一种新型透明聚合物涂层的商业化，该涂层由一种无刺痛、挥发性疏水溶剂提供，用于治疗一、二度烧伤，减少疤痕形成。烧伤是医疗保健中最昂贵的方面之一。全世界每年有4000多万疤痕患者，治疗费用为120亿美元。目前，没有商业烧伤护理产品或方法提供改善烧伤愈合，减少疼痛和减少疤痕形成利用病人和护理人员友好的技术，如本研究给出。尽管组织工程在制备用于局部伤口护理的支架方面进行了大量研究，但少数商业化产品在制备、储存和使用方面成本高昂，并且容易降解和丧失活性。随着该项目的成功，一项新技术将被开发出来，它将显著改善烧伤治疗，该技术是基于在商业溶剂中容易合成的稳定聚合物，它提供了一种透明的、不溶于水的、透氧和透水蒸气的涂层，适用于大面积烧伤创面，而不会形成修补设计，并且随着创面愈合而自我去除。

This Small Business Innovation Research (SBIR) Phase II project pertains to the development of a spray-on polymer coating using a novel amphiphilic polymer in a non-stinging, volatile hydrophobic solvent for treatment of first and second degree burn wounds with reduction of scar formation, wherein a transparent, flexible substrate for human cell attachment, viability and proliferation is formed without any added exogenous biological components. No methods currently exist for treating burns that concurrently reduce pain upon treatment, are intimately conformal to regular and irregular (face, fingers) wound surfaces, provide a cell substrate for wound closure and healing, and do not require dressing removal, such as with the proposed spray-on polymer coating. This investigation will involve cytocompatibility and wound healing studies of these novel polymers, in conjunction with a determination of the polymers? physical and mechanical properties needed for spray-on characteristics. The success of this investigation will demonstrate that a sophisticated, yet inexpensive, easily applied, conformal polymer coating can be used for burn treatment with reduction of scarring. The broader impact/commercial potential of this project pertains to the commercialization of a novel spray-on transparent polymer coating, delivered from a non-stinging, volatile hydrophobic solvent, to treat first and second degree burn injuries, with reduction of scar formation. Burn injuries constitute one of the most expensive aspects of health care. There are more than 40 million scar patients worldwide per year, at a treatment cost of $12 billion. Currently, no commercial burn care product or methodology provides for improved burn healing with reduced pain and reduced scar formation utilizing a patient and caregiver-friendly technique such as given by this investigation. Despite substantial research in tissue engineering for the preparation of scaffolds for topical wound care, the few commercialized products are costly in preparation, storage and use, and are subject to degradation and loss of activity. With the success of this project, a new technology will be developed that significantly improves burn treatment, is based upon readily synthesized, stable polymers in a commercially-available solvent, that provides a transparent, water-insoluble, oxygen- and water-vapor permeable coating suitable for use over large area burn wounds without forming a patch-work design, and which self-removes as the wound heals.