Synthesizing Polyhydroxyalkanoates from Fermented Dairy Manure

1235885(外套)。本研究将系统地将蛋白质组学方法与环境工程原理相结合，以推进废物转化为产品的过程。将有机废物升级为可生物降解塑料的过程，聚羟基烷酸酯（pha）。这一过程不仅能将原本作为温室气体排放的碳封存起来，而且高价值产品（PHA）将取代石油的使用。PHA技术为工业生产具有经济价值的产品提供了机会，同时降低了废物管理成本。本研究试图回答的首要基础研究问题如下：？什么是与混合微生物群落合成盛宴/饥荒PHA相关的控制代谢/蛋白质？如何使用标准生物反应器操作标准对它们进行功能操作，以最大限度地将VFAs转化为PHA？以下研究问题将指导该项目。rq1：奶牛营养对挥发性脂肪酸合成的潜在影响是什么？rq2：与有氧盛宴/饥荒PHA合成相关的代谢特异性蛋白是什么？潜在的基因调控因子是什么？rq3：在不同的微生物物种中，进行盛宴/饥荒PHA合成的代谢特异性蛋白质和调节因子是相同的还是相似的？rq4：我们如何应用和操作传统的生物反应器操作标准，以最大限度地富集盛宴/饥荒PHA微生物，并诱导必要的代谢，以产生最大的VFA作为PHA的隔离？研究方法概述如下。实验1将评价和确定粪便特性对VFA合成的影响。对象2和3活动是本研究的核心焦点，将响应rq 2-4。该研究将整合在盛宴/饥荒PHA合成的混合微生物群体中精确的宏观/微观结构功能关系。项目2和3的结果将在分子结构/功能水平上确定和解释生物反应器操作标准，以一种传统的方式和水平最大化PHA合成。黑盒吗?方法无法定义。这项研究的一个重要成果将是在项目4下进行中试规模的工艺验证调查。本研究将为牛粪生物转化为高经济价值生物塑料（PHA）提供基础知识和实践知识。该项目将教育西班牙裔高中生，并为西班牙裔大学生提供研究机会。

1235885 (Coats). This research will systematically integrate proteomic methods with environmental engineering principles to advance a ?waste-to-products? process to upcycle organic waste to a biodegradable plastic, polyhydroxyalkanoates (PHAs). Not only will the process sequester carbon that would otherwise be emitted as a greenhouse gas, the high-value product (PHA) will displace petroleum usage. The PHA technology represents opportunities for industries to generate products of economic value while reducing waste management costs. The overarching fundamental research question this proposed study seeks to answer is as follows: ?What are the controlling metabolisms/proteins associated with feast/famine PHA synthesis by mixed microbial consortia and how can they be functionally manipulated using standard bioreactor operating criteria to maximize conversion of VFAs to PHA?? The following research questions will guide the project. RQ 1: What are the potential effects of dairy cow nutrition on volatile fatty acid synthesis? RQ 2: What are the metabolism-specific proteins associated with aerobic feast/famine PHA synthesis? What are the underlying genetic regulators? RQ 3: Are the metabolism-specific proteins and regulators identical, or similar, across different microbial species performing feast/famine PHA synthesis? RQ 4: How do we apply and manipulate traditional bioreactor operating criteria to maximize enrichment of feast/famine PHA microbes and induce necessary metabolisms to yield maximum VFA sequestration as PHA? An overview of the research approach is as follows. Obj. 1 activities will evaluate and establish the effects of manure characteristics on VFA synthesis. Obj. 2 and 3 activities are the core focus of this research that will respond to RQs 2-4. The research will integrate to exact macro/micro structure-function relationships on mixed microbial consortia performing feast/famine PHA synthesis. Results from Obj. 2 and 3 will identify and explain at a molecular structure/function level the bioreactor operating criteria necessary to maximize PHA synthesis in a manner and at a level that traditional ?black box? methods could not define. An important culmination of this research will be the pilot-scale process validation investigations under Obj. 4. This research will advance both fundamental and practical knowledge to bio-convert dairy manure to high economic value bioplastic (PHA). The project will educate Hispanic high school students and provide research opportunities to undergraduate Hispanic students.