NSF/FDA SIR: Biomaterials for MSC Adhesion and Enrichment

间充质干细胞(MSCs)具有向骨、脂肪和软骨等多种间充质细胞分化的能力，具有巨大的治疗潜力。然而，MSCs只代表了骨髓中发现的细胞的一小部分，并且缺乏唯一的识别标记，这证明它们很难准确分离。传统的骨髓间充质干细胞黏附有几个局限性，包括骨髓间充质干细胞与骨髓内其他细胞类型之间潜在的有害相互作用，后期黏附的骨髓间充质干细胞可能丢失，以及在骨髓中发现的非骨髓间充质干细胞黏附细胞的不受欢迎的扩张。这项提议的目标有两个。首先，通过设计一种独特的材料来克服传统塑料黏附的局限性，这种材料具有可调的机械和表面特性，能够在不同种类的人群中强烈和选择性地黏附MSCs。第二，在相同的材料上培养和分化丰富的捕获的MSC种群。

ABSTRACT #1238398Fisher, John P.Mesenchymal stem cells (MSCs) have tremendous therapeutic potential as they are capable of differentiating into various mesenchymal lineages such as bone, adipose, and cartilage. However, MSCs represent only a fraction of the cells that are found in the bone marrow and lack unique identifying markers, proving them difficult to isolate precisely. There are several limitations to traditional plasticadherence, including potentially harmful interactions between MSCs and the other cell types within the bone marrow, possible loss of late-adhering MSCs, and the undesired expansion of non-MSC adherent cells found in the bone marrow. The goal of this proposal is two-fold. First to overcome the limitations of traditional plastic adherence by designing a unique material with tuned mechanical and surface properties to strongly and selectively adhere MSCs in a heterogeneous population. Second, to culture and differentiate the enriched captured MSC population on the same material.