NSF/FDA SIR: Biomaterials for MSC Adhesion and Enrichment

NSF/FDA SIR：用于 MSC 粘附和富集的生物材料

• 批准号: 1238398
• 负责人: John Fisher
• 金额: $ 12.26万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1238398&HistoricalAwards=false
• 关键词: NSF; FDA; SIR; Biomaterials; MSC

ABSTRACT #1238398Fisher, John P.Mesenchymal stem cells (MSCs) have tremendous therapeutic potential as they are capable of differentiating into various mesenchymal lineages such as bone, adipose, and cartilage. However, MSCs represent only a fraction of the cells that are found in the bone marrow and lack unique identifying markers, proving them difficult to isolate precisely. There are several limitations to traditional plasticadherence, including potentially harmful interactions between MSCs and the other cell types within the bone marrow, possible loss of late-adhering MSCs, and the undesired expansion of non-MSC adherent cells found in the bone marrow. The goal of this proposal is two-fold. First to overcome the limitations of traditional plastic adherence by designing a unique material with tuned mechanical and surface properties to strongly and selectively adhere MSCs in a heterogeneous population. Second, to culture and differentiate the enriched captured MSC population on the same material.

摘要＃1238398鱼类，John P.Mespsidemal干细胞（MSC）具有巨大的治疗潜力，因为它们能够区分各种间充质谱系，例如骨骼，脂肪和软骨。但是，MSC仅代表骨髓中发现的细胞的一部分，并且缺乏唯一的识别标记，证明它们很难精确地隔离。传统的塑料疗法有几个局限性，包括MSC与骨髓中其他细胞类型之间的潜在有害相互作用，可能会损失晚期粘附MSC，以及在骨髓中发现的非MSC粘附细胞的不希望扩张。该提议的目标是两个方面。首先，通过设计具有调谐的机械和表面特性的独特材料来克服传统塑料依从性的局限性，以在异质种群中强烈有选择地遵守MSC。其次，在同一材料上培养和区分富集的捕获的MSC种群。