High resolution Imaging Mass Spectrometry (IMS) of neuropeptides in dynamic systems

动态系统中神经肽的高分辨率成像质谱 (IMS)

• 批准号: 208521253
• 负责人: Professor Dr. Reinhard Predel
• 金额: --
• 依托单位: Institut für Zoologie
• 依托单位国家: 德国
• 项目类别: Major Instrumentation Initiatives
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/208521253?language=en
• 关键词: resolution; Imaging; Mass; Spectrometry; IMS

The purpose of the project is the development of a MSI-platform for high resolution analysis of spatial localization and dynamics of the structurally and functionally most variable messenger molecules in the CNS, namely neuropeptides. While the new field of neuropeptidomics allows the neuropeptides from a sample to be characterized, their spatial dynamics are poorly understood because of a lack of measurement tools. There is a critical need for improved mass-spectrometrybased imaging/localization. The maintenance of a sufficient resolution in combination with high sensitivity as typical of conventional direct tissue profiling is a challenge in MSI of neuropeptides. The applicants cover longstanding experience in Biochemistry, Neuroscience, and Genetics. M most importantly in the context of the proposal, they have a strong background in MALDI-TOF mass spectrometry and peptidomics, and focused on the development of methods for the analysis of neuropeptides in identified and dissected neurons, in Drosophila and other insect nervous and peripheral tissue or method development in MSI itself (Sweedler). The proposal is divided in two subprojects: 1) Development of MSI for the analysis of immediate compensation mechanisms within the mammalian peptidergic system. 2) Development of MSI-approaches for the functional analysis of neuropeptide dynamics in development and behaviour of a social insect.

该项目的目的是开发一个MSI平台，用于高分辨率分析CNS中结构和功能最可变的信使分子（即神经肽）的空间定位和动力学。虽然神经肽组学的新领域允许从一个样本的神经肽的特点，他们的空间动力学知之甚少，因为缺乏测量工具。迫切需要改进的基于质谱的成像/定位。维持足够的分辨率与高灵敏度的组合，作为传统的直接组织分析的典型是一个挑战，在MSI的神经肽。申请人涵盖生物化学，神经科学和遗传学的长期经验。M最重要的是，在该提案的背景下，他们在MALDI-TOF质谱和肽组学方面有很强的背景，并专注于开发用于分析鉴定和解剖神经元中的神经肽的方法，在果蝇和其他昆虫神经和外周组织中或MSI本身的方法开发（Sweedler）。该提案分为两个子项目：1）开发MSI，用于分析哺乳动物肽能系统内的即时补偿机制。2)社会昆虫发育和行为中神经肽动力学功能分析的MSI方法的发展。