Multimodal Imaging Mass Spectrometry and Spatial Omics for the Human Kidney
人类肾脏的多模态成像质谱和空间组学
基本信息
- 批准号:10701835
- 负责人:
- 金额:$ 61.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-15 至 2027-06-30
- 项目状态:未结题
- 来源:
- 关键词:Academic Medical CentersAcute Renal Failure with Renal Papillary NecrosisAffectAgeAnalytical ChemistryAnatomyAtlasesAutomobile DrivingBiologicalBiopsyBlood capillariesCellsCellular biologyCharacteristicsChronic Kidney FailureClinicalCollectionComputer AnalysisDataData AnalysesData ScienceData SetDigestionDiseaseDistalDuct (organ) structureElementsEnvironmentFiltrationHeterogeneityHomeostasisHumanHuman BioMolecular Atlas ProgramImageImage AnalysisIndividualInfrastructureKidneyKidney DiseasesKnowledgeLabelLaboratoriesLearningLinkLipidsLymphaticMapsMass Spectrum AnalysisMessenger RNAMicroscopyMissionModalityMolecularMolecular AnalysisMolecular ProfilingMorphologyMultimodal ImagingNeighborhoodsNephrologyNephronsPathologyPathway interactionsPatientsPerformanceProcessProteinsProteomicsProtocols documentationRaceResearchResearch PersonnelResolutionResourcesSamplingSiteSodium ChlorideSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStainsStructureSurfaceTechnologyTherapeutic InterventionTissue atlasTissuesTranslatingTubular formationUniversitiesValidationWateranalysis pipelinebiocomputingblood pressure controlcell typeclinical phenotypecohortcomputerized toolsdata integrationdigitaldisease heterogeneitydisorder subtypeexperienceexperimental studyextracellularhuman imagingimage registrationimaging Segmentationimaging facilitiesinjuredinnovationinsightinterstitialkidney biopsykidney cellkidney imaginglaser capture microdissectionmass spectrometric imagingmolecular imagingmultimodal datamultimodalitymultiple omicsnano-stringnext generation sequencingpatient populationpotential biomarkerprecision medicinesexspatial integrationspatial relationshipspecific biomarkerssupervised learningtranscriptomicsunsupervised learning
项目摘要
PROJECT SUMMARY: The mission of the proposed Kidney Precision Medicine Project Tissue Interrogation
Site (TIS) is to apply a platform of integrated multiomic imaging and spatially resolved molecular technologies to
biopsies from patients with acute kidney injury (AKI) and chronic kidney disease (CKD) to define key pathways
and understand the molecular drivers of disease heterogeneity. This TIS will leverage the unique resources of
the Mass Spectrometry Research Center and VU Biomolecular Imaging Center, as well as the world-class clinical
environment of the Vanderbilt University Medical Center, the valuable input of our patient partner, and the
advanced biocomputational infrastructure available through the data analysis laboratories at Vanderbilt
University and the Delft University of Technology. The main objectives of our TIS are to: (1) generate high quality
images of kidney biopsies using an innovative state-of-the-art multimodal imaging pipeline that includes matrix-
assisted laser desorption/ionization imaging mass spectrometry (IMS), autofluorescence, and stained
microscopy; (2) acquire spatially resolved proteomics and transcriptomics data using microLESA, laser capture
microdissection, and the GeoMx Digital Spatial Profiler as well as perform network and enrichment analysis to
validate and enhance biological interpretation of the imaging data; and (3) implement advanced data analysis
strategies to identify potential biomarkers and optimal points of therapeutic intervention. With this suite of
technologies, data analysis capabilities, and previous experience developing atlases of healthy-for-age human
kidney tissue, we will create kidney tissue atlases to define the molecular landscape of biopsies from various
patient populations (e.g., age, race, sex) as well as of AKI and CKD clinical phenotypes, disease states, and
transitions; with an eye toward determining potential disease subclasses. To accomplish these aims, we
assembled a highly interactive and established team of investigators consisting of complementary expertise in
nephrology, cell biology, analytical chemistry, and data science.
项目总结:拟议肾脏精准医学项目组织询问的使命
研究中心(TIS)将应用集成多组成像和空间分辨分子技术的平台,
急性肾损伤(阿基)和慢性肾病(CKD)患者的活检,以确定关键途径
并了解疾病异质性的分子驱动因素。该TIS将利用
质谱研究中心和VU生物分子成像中心,以及世界一流的临床
范德比尔特大学医学中心的环境,我们的患者合作伙伴的宝贵投入,以及
范德比尔特的数据分析实验室提供先进的生物计算基础设施
大学和德尔夫特理工大学。我们的TIS的主要目标是:(1)产生高质量
使用创新的最先进的多模式成像管道,包括矩阵,
辅助激光解吸/电离成像质谱(IMS)、自体荧光和染色
(2)使用microLESA、激光捕获和激光显微镜获取空间分辨蛋白质组学和转录组学数据
显微解剖和GeoMx数字空间剖面仪,以及执行网络和富集分析,
验证和增强成像数据的生物学解释;以及(3)实施高级数据分析
确定潜在的生物标志物和治疗干预的最佳点的策略。有了这套
技术,数据分析能力,以及以前开发老年人健康地图集的经验
肾组织,我们将创建肾组织图谱,以定义各种活检的分子景观
患者群体(例如,年龄、种族、性别)以及阿基和CKD临床表型、疾病状态和
过渡;着眼于确定潜在的疾病亚类。为了实现这些目标,我们
组建了一个高度互动和成熟的调查小组,
肾脏学、细胞生物学、分析化学和数据科学。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jeffrey M Spraggins其他文献
Jeffrey M Spraggins的其他文献
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{{ truncateString('Jeffrey M Spraggins', 18)}}的其他基金
Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Mapping of the Human Kidney
范德比尔特大学生物分子多模态成像中心进行人体肾脏 3 维绘图
- 批准号:
10530867 - 财政年份:2022
- 资助金额:
$ 61.25万 - 项目类别:
Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Mapping of the Human Kidney
范德比尔特大学生物分子多模态成像中心进行人体肾脏 3 维绘图
- 批准号:
10701832 - 财政年份:2022
- 资助金额:
$ 61.25万 - 项目类别:
Multimodal Imaging Mass Spectrometry and Spatial Omics for the Human Kidney
人类肾脏的多模态成像质谱和空间组学
- 批准号:
10515051 - 财政年份:2022
- 资助金额:
$ 61.25万 - 项目类别: