Mechanisms of Acquired Immunity in Bacteria
细菌获得性免疫的机制
基本信息
- 批准号:1244557
- 负责人:
- 金额:$ 45.65万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-01-15 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Title: Mechanism of Acquired Immunity in BacteriaSenior personnel: Jennifer Doudna, University of California at Berkeley (Principle investigator)Scientific research: Control of viral infections in bacterial populations is critical to the use of microbes for a wide variety of applications including bioremediation and biofuel production. The discovery of RNA-based acquired immunity in bacteria, mediated by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs), provides an exciting opportunity to exploit natural pathways for regulating gene expression if the underlying molecular mechanisms can be discovered and manipulated. This project aims to determine how self versus non-self recognition is achieved, how new DNA sequences are acquired during viral infections and used to immunize cells against future infections. This project has the potential to transform the way we understand gene regulation in bacteria, and to reveal the molecular basis for a previously unknown and unanticipated mechanism of anti-viral defense. To understand the independent evolution and physiological functions of bacterial pathways utilizing RNA molecules to control genetic elements, it will be necessary to determine the molecular mechanisms of CRISPR activity. The potential impact of this project includes both discovering the mechanisms by which microbes acquire immunity to viruses, and providing the knowledge necessary to manipulate or engineer those systems for various applications. The project provides an exciting avenue of research for students, and is particularly appealing to undergraduates who are being exposed to protein and RNA biochemistry for the first time. There are ample opportunities for students to make truly new discoveries in this field, with potentially very high impact.Broader impacts: The primary investigator's lab has a successful history of training students at all levels in the creative dissection of molecular mechanisms involving RNA and RNA-protein complexes using a variety of experimental methods. The project will be conducted in a highly collaborative environment at UC Berkeley, which has become a de facto center for CRISPR research encompassing ongoing work in the laboratories of Jill Banfield, Phil Hugenholtz and Adam Arkin. An annual CRISPR conference attracts scientists from around the world to UC Berkeley to share their latest results. Students attend and present their work at this meeting, providing an outstanding opportunity for learning and discussing new results.
职务名称:细菌获得性免疫的机制高级人员:Jennifer Doudna,加州大学伯克利分校(首席研究员)科学研究:控制细菌种群中的病毒感染对于微生物用于各种应用(包括生物修复和生物燃料生产)至关重要。在细菌中发现基于RNA的获得性免疫,由重复的规则间隔短回文重复序列(CRISPR)介导,如果可以发现和操纵潜在的分子机制,则为利用天然途径调节基因表达提供了一个令人兴奋的机会。该项目旨在确定自我与非自我识别是如何实现的,新的DNA序列是如何在病毒感染过程中获得的,并用于免疫细胞对抗未来的感染。该项目有可能改变我们理解细菌基因调控的方式,并揭示以前未知和未预料到的抗病毒防御机制的分子基础。为了了解利用RNA分子控制遗传元件的细菌途径的独立进化和生理功能,有必要确定CRISPR活性的分子机制。该项目的潜在影响包括发现微生物获得病毒免疫力的机制,并提供操纵或设计这些系统以用于各种应用所需的知识。该项目为学生提供了一个令人兴奋的研究途径,特别是吸引那些第一次接触蛋白质和RNA生物化学的本科生。学生有充分的机会在这个领域做出真正的新发现,具有潜在的非常高的影响。更广泛的影响:主要研究者的实验室有一个成功的历史,培训学生在各个层次的创造性解剖分子机制,涉及RNA和RNA-蛋白质复合物使用各种实验方法。该项目将在加州大学伯克利分校的一个高度协作的环境中进行,该环境已成为CRISPR研究的事实中心,包括Jill Banfield,Phil Hugenholtz和Adam Arkin实验室正在进行的工作。一年一度的CRISPR会议吸引了来自世界各地的科学家到加州大学伯克利分校分享他们的最新成果。学生参加并在本次会议上介绍他们的工作,为学习和讨论新的成果提供了一个很好的机会。
项目成果
期刊论文数量(0)
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Jennifer Doudna其他文献
Mechanistic basis of atypical TERT promoter mutations
非典型 TERT 启动子突变的机制基础
- DOI:
10.1038/s41467-024-54158-5 - 发表时间:
2024-11-18 - 期刊:
- 影响因子:15.700
- 作者:
Kerryn Elliott;Vinod Kumar Singh;Alan Bäckerholm;Linnea Ögren;Markus Lindberg;Katarzyna M. Soczek;Emily Hoberg;Tom Luijts;Jimmy Van den Eynden;Maria Falkenberg;Jennifer Doudna;Anders Ståhlberg;Erik Larsson - 通讯作者:
Erik Larsson
Jennifer Doudna的其他文献
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{{ truncateString('Jennifer Doudna', 18)}}的其他基金
Collaborative Research: TRTech-PGR TRACK: Discovery and characterization of small CRISPR systems for virus-based delivery of heritable editing in plants.
合作研究:TRTech-PGR TRACK:小型 CRISPR 系统的发现和表征,用于基于病毒的植物遗传编辑传递。
- 批准号:
2334028 - 财政年份:2024
- 资助金额:
$ 45.65万 - 项目类别:
Standard Grant
I-Corps: Curing inherited diseases at the source through next-generation clustered regularly interspaced short palindromic repeat (CRISPR) systems
I-Corps:通过下一代簇状规则间隔短回文重复 (CRISPR) 系统从源头治愈遗传性疾病
- 批准号:
2227919 - 财政年份:2022
- 资助金额:
$ 45.65万 - 项目类别:
Standard Grant
Mechanism of Acquired Immunity in Bacteria
细菌获得性免疫的机制
- 批准号:
1817593 - 财政年份:2018
- 资助金额:
$ 45.65万 - 项目类别:
Standard Grant
Mechanism of Acquired Immunity in Bacteria
细菌获得性免疫的机制
- 批准号:
0950971 - 财政年份:2010
- 资助金额:
$ 45.65万 - 项目类别:
Continuing Grant
National Science Foundation: Alan T. Waterman Award
美国国家科学基金会:艾伦·T·沃特曼奖
- 批准号:
0244319 - 财政年份:2002
- 资助金额:
$ 45.65万 - 项目类别:
Continuing Grant
National Science Foundation: Alan T. Waterman Award
美国国家科学基金会:艾伦·T·沃特曼奖
- 批准号:
0003240 - 财政年份:2000
- 资助金额:
$ 45.65万 - 项目类别:
Continuing Grant
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