Kinetically-stabilized mesoscopic protein aggregates

动力学稳定的介观蛋白质聚集体

• 批准号: 1244568
• 负责人: Vassiliy Lubchenko
• 金额: $ 60万
• 依托单位: University of Houston
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244568&HistoricalAwards=false
• 关键词: Kinetically; stabilized; mesoscopic; protein; aggregates

It is generally accepted that a protein's amino acid sequence is optimized so that the molecule consistently folds into a unique, stable 3D shape while avoiding aggregation with other biomolecules. Yet this view is overly simplistic. On the one hand, many proteins are not monolithic structures; for instance, myoglobin must reliably deform to let in oxygen. On the other hand, under certain conditions, proteins may aggregate into amyloid fibers while in a misfolded conformation. Although very complex, these phenomena can still be understood thermodynamically. Proteins are deformable given a sufficiently large native state entropy, while amyloid fibers are stabilized by multiple amino acid contacts within the fiber. Much less understood are the mesoscopic liquid-like aggregates recently found in several protein solutions, including lysozyme, hemoglobin, and gamma-crystallin. These clusters are important as nucleation sites for protein crystals. The objective of this project is to test a microscopic hypothesis for the formation of the mesoscopic clusters by which the clusters result from the formation of long-lived protein complexes stabilized at high densities. The unusually large cluster size stems from the long lifetime of the complexes, which in turn owe their stability to the conformational freedom of constituent protein molecules. A key component of the proposed mechanism is that proteins partially unfold prior to binding and may even undergo domain swapping, whereby protein segments bind to complimentary segments on a different protein. The PIs will employ a combination of dynamic light scattering, Brownian microscopy, amide exchange NMR, and theoretical modeling to test this mechanism in several systems, including proteins barnase, RNase A, hemoglobin, insulin, and lysozyme.This research is a multidisciplinary effort by a theoretical physical chemist and experimental chemical engineer. A synthesis of a wide range of physicochemical and biochemical measurements and physical modeling will be undertaken to tackle this difficult problem; the outcome is expected to produce fundamental understanding of biological processes and provide new avenues for making new materials. Graduate, undergraduate, and high school students will be trained at the interface between physics, chemistry, and biology. The home institution of the PIs, the University of Houston, is one of the most ethnically diverse research universities in the nation, and is one of only three Hispanic-Serving Institutions in the US. Existing collaborations with local writers and radio personalities will be utilized to publicize the societal benefits of the research. This project is jointly supported by the Molecular Biophysics Cluster in the Division of Molecular and Cellular Biosciences and the Physics of Living Systems Program in the Physics Division.

一般认为，蛋白质的氨基酸序列是优化的，使得分子一致地折叠成独特的、稳定的3D形状，同时避免与其他生物分子聚集。 然而，这种观点过于简单化了。 一方面，许多蛋白质不是整体结构;例如，肌红蛋白必须可靠地变形以让氧气进入。 另一方面，在某些条件下，蛋白质可以聚集成淀粉样纤维，同时处于错误折叠的构象。 虽然这些现象非常复杂，但仍然可以从理论上加以理解。 蛋白质在足够大的自然状态熵下是可变形的，而淀粉样蛋白纤维通过纤维内的多个氨基酸接触而稳定。 最近在几种蛋白质溶液中发现的介观液体状聚集体，包括溶菌酶，血红蛋白和γ-晶状体蛋白，了解得少得多。 这些团簇作为蛋白质晶体的成核位点是重要的。 该项目的目的是测试一个微观假设的介观集群的形成，集群的结果从形成长寿命的蛋白质复合物稳定在高密度。 异常大的簇大小源于复合物的长寿命，这反过来又将其稳定性归功于组成蛋白质分子的构象自由。 所提出的机制的一个关键组成部分是蛋白质在结合之前部分解折叠，甚至可能进行结构域交换，从而蛋白质片段与不同蛋白质上的互补片段结合。 PI将采用动态光散射，布朗显微镜，酰胺交换NMR和理论建模的组合，以测试在几个系统，包括蛋白质芽孢杆菌RNA酶，RNA酶A，血红蛋白，胰岛素和溶菌酶的这种机制。这项研究是一个多学科的努力，由理论物理化学家和实验化学工程师。 将进行广泛的物理化学和生物化学测量和物理建模的综合，以解决这个难题;预计结果将产生对生物过程的基本理解，并为制造新材料提供新的途径。 研究生，本科生和高中生将在物理，化学和生物学之间的接口进行培训。 PI的家乡机构，休斯顿大学，是全国最具种族多样性的研究型大学之一，也是美国仅有的三个西班牙裔服务机构之一。 将利用与当地作家和广播界人士的现有合作，宣传研究的社会效益。 该项目由分子和细胞生物科学部的分子生物物理学集群和物理学部的生命系统物理学项目共同支持。