Assembly and Function of the Cyanobacterial Photosystem II Complex

蓝藻光系统 II 复合体的组装和功能

• 批准号: 1244586
• 负责人: Robert Burnap
• 金额: $ 72.73万
• 依托单位: Oklahoma State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244586&HistoricalAwards=false
• 关键词: Assembly; Function; Cyanobacterial; Photosystem; II

Intellectual Merit: If carbon-neutral energy and chemical feedstock solutions are to be developed, these will likely include natural or engineered photosynthetic organisms that manufacture valuable products and/or engineered devices that mimic the principals of natural photosynthesis by coupling of light capture to charge separation and then to endergonic chemical bond rearrangement. An understanding of oxygenic photosynthesis is important on both counts. Despite much progress, there are many important facets of the photosynthetic mechanism that remain to be understood. Of central importance is the water oxidation mechanism catalyzed by photosystem II (PSII), which utilizes water as the source of reductant to fix inorganic carbon. Many aspects of the enzyme's oxygen-yielding water oxidation mechanism remain poorly understood. This includes the repair processes necessitated by incessant photodamage sustained by PSII. These photodamage processes are due to the extraordinary high-potential multi-electron redox reactions that PSII catalyzes; also an important consideration for natural and biomimetic solar energy applications. This proposal addresses two major questions: First, is there a signal conveying the occurrence of damage to an individual PSII complex thereby targeting it for repair or is the repair process a generalized response randomly operating on the population of PSII in response to photodamage-inducing conditions? Second, how are the active site metals, manganese and calcium, assembled into a catalytically active complex? The project will combine molecular genetic, biochemical and biophysical approaches to understand the D1 protein repair process and the assembly and function of the catalytically active complex and also the role of the proteins that tune its reactivity and organize its structure.Broader Impacts: Immersing high school science teachers in a research environment that will be combined with professional development activities will help educators gain an explicit view of how scientific knowledge is generated and will also help with their own curriculum development. Teacher participants will be trained to conduct research and mentor students. They will perpetuate this acquired knowledge with their students by implementing new content knowledge and pedagogical skills into their existing curriculum, mentoring students in student research projects and coaching students in science fair competitions. During state and national conferences teachers will share their developed curriculum and mentoring skills with other educators. The PI and Co-PI will disseminate the science research findings and the educational outreach experience at science and education conference, presenting at the OSU National Lab Day, and through research and practitioner journals.

智力优势：如果要开发碳中性能源和化学原料解决方案，这些解决方案可能包括自然或工程光合作用生物，这些生物生产有价值的产品和/或工程设备，通过耦合光捕获、电荷分离和增强化学键重排来模拟自然光合作用的原理。对产氧光合作用的理解在这两个方面都很重要。尽管取得了很大进展，但光合作用机制的许多重要方面仍有待了解。最重要的是光系统II(PSII)催化的水氧化机制，它利用水作为还原剂来固定无机碳。该酶的产氧水氧化机制的许多方面仍然知之甚少。这包括PSII遭受的持续光损伤所必需的修复过程。这些光损伤过程是由于PSII催化的超高电势多电子氧化还原反应；也是自然和仿生太阳能应用的重要考虑因素。这一建议解决了两个主要问题：第一，是否存在一个信号来传递单个PSII复合体的损伤发生，从而将其作为修复的目标，或者修复过程是对PSII种群的随机反应，以响应光损伤诱导条件？第二，活性中心金属锰和钙是如何组装成具有催化活性的络合物的？该项目将结合分子遗传学、生化和生物物理方法，了解D1蛋白质修复过程和催化活性复合体的组装和功能，以及调节其反应性和组织其结构的蛋白质的作用。广泛影响：将高中科学教师沉浸在与专业发展活动相结合的研究环境中，将有助于教育工作者明确了解科学知识是如何产生的，也将有助于他们自己的课程开发。教师参与者将接受培训，以进行研究和指导学生。他们将通过将新的教学知识和教学技能应用到现有课程中，在学生研究项目中指导学生，并在科学博览会比赛中指导学生，使这些已获得的知识在学生中永久化。在州和全国会议期间，教师将与其他教育工作者分享他们开发的课程和指导技能。国际和平协会和联合国际将在科学和教育会议上传播科学研究成果和教育推广经验，在俄亥俄州立大学国家实验室日上发表报告，并通过研究和实践者期刊。