Development of Gas-Phase Biomolecule Ion / Radical Reactions on a Linear Ion Trap Mass Spectrometer

化学测量和成像（CMI）计划的这一奖项支持普渡大学Yu Xia教授的工作，以开发和验证用于在线性离子阱质谱（MS）系统上实施生物分子离子/自由基反应的仪器。 脉冲热解喷嘴将用于形成强自由基束并将其转移到线性离子阱，其中肽离子被存储用于反应。 将通过二硫键连接的肽离子与烯丙基或苯基自由基之间的反应测试改良仪器的性能。 这是一个高风险/高回报的项目，其中有效地形成自由基并将其转移到高真空中是一项具有挑战性的任务。然而，所学到的知识不仅能够更好地理解生物分子离子/自由基反应，而且还提供了一个可行的平台，以研究对燃烧过程和大气化学重要的其他气相反应。拟议的研究和教育活动强调综合基础科学研究和仪器的作用，为分析化学的研究生和本科生建立了良好的培训基地。女性和少数民族学生将在该计划中得到指导，并参加普渡大学AGEP（中西部研究生教育和教授十字路口联盟）活动。

This award by Chemical Measurement and Imaging (CMI) program supports work by Professor Yu Xia at Purdue University to develop and validate instrumentation for implementing biomolecule ion/radical reactions on a linear ion trap mass spectrometry (MS) system. A pulsed pyrolysis nozzle will be used to form and transfer intense radical beams to a linear ion trap, where peptide ions are stored for reactions. The performance of the modified instrument will be tested from reactions between disulfide linked peptide ions and allyl or phenyl radicals. It is a high risk/high reward project where efficient formation and transfer of radicals into high vacuum is a challenging task. Yet the learned knowledge will not only enable better understanding of biomolecular ion/radical reactions, but also provide a viable platform to study other gas-phase reactions important to combustion processes and atmospheric chemistry. The proposed research and education activities emphasize the role of integrated fundamental scientific research and instrumentation, which establishes excellent training ground for both graduate and undergraduate students in analytical chemistry. Female and minority students will be mentored in this program and be participating in Purdue AGEP (Midwest Crossroads Alliance for Graduate Education and the Professoriate) events.