Gas-Phase Ion Chemistry of Peptide Radical Ions and Applications in Protein Characterization

肽自由基离子的气相离子化学及其在蛋白质表征中的应用

• 批准号: 1308114
• 负责人: Yu Xia
• 金额: $ 37.1万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1308114&HistoricalAwards=false
• 关键词: Gas; Phase; Ion; Chemistry; Peptide

With support from the Chemical Measurement and Imaging (CMI) program in the Division of Chemistry, Professor Yu Xia and her group at Purdue University expand the capability of mass spectrometry to study radical ions derived from biomolecules, which are significantly under-explored as compared to even electron ions. New methods and instrumentation will be developed to form diverse types of peptide radical ions in gas-phase and extract their chemical property information including structures and intrinsic reactivity. New analytical approaches based on peptide radical ion chemistry will be explored for protein structural characterization. Professor Xia proposes to study how radicals and ions interact with each other. The gained knowledge will provide insight on the fate of protein radical intermediates resulted from oxidative stress, which has been shown to be related to various biological processes, including aging and neurodegeneration. The proposed research and education activities will also establish fertile training ground for both graduate and undergraduate students with exposure to scientific instrumentation, reaction mechanisms, and bio-analysis. Broadening participation of female students and students from underrepresented groups will be one of the focuses of the proposed educational component.

在化学系化学测量和成像（CMI）计划的支持下，普渡大学的Yu Xia教授和她的团队扩展了质谱法研究来自生物分子的自由基离子的能力，与电子离子相比，这些自由基离子的探索程度显着不足。 新的方法和仪器将被开发，以形成不同类型的肽自由基离子在气相中，并提取其化学性质的信息，包括结构和内在的反应性。 将探索基于肽自由基离子化学的新分析方法用于蛋白质结构表征。 夏教授建议研究自由基和离子如何相互作用。所获得的知识将提供对由氧化应激引起的蛋白质自由基中间体的命运的见解，氧化应激已被证明与各种生物过程有关，包括衰老和神经退行性变。拟议的研究和教育活动还将为研究生和本科生建立肥沃的培训基地，接触科学仪器，反应机制和生物分析。扩大女生和代表性不足群体的学生的参与将是拟议教育部分的重点之一。