CAREER: Increasing the power of single-molecule bio-imaging with plasmon-enhanced fluorescence

职业：通过等离子体增强荧光增强单分子生物成像的能力

• 批准号: 1252322
• 负责人: Julie Biteen
• 金额: $ 51.3万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1252322&HistoricalAwards=false
• 关键词: CAREER; Increasing; power; single; molecule

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Julie Biteen and her group at the University of Michigan are improving the power of microscopes in order to look inside live cells at the 1-2 nanometer scale of proteins themselves. This project is based on coupling single-molecule fluorescence microscopy and metal nanoparticle plasmonics to produce a non-perturbative method for looking at protein position and dynamics in the natural cellular environment with unprecedented resolution and flexibility.The interdisciplinary approach aims (1) to understand the mechanism of plasmon-enhanced single-molecule fluorescence, (2) to characterize plasmonic surfaces based on this insight, (3) to localize and track membrane proteins in live bacteria cells coupled to metal nanoparticles, and (4) to expose students to modern science through demos, mentoring, teaching and laboratory research, with a particular eye toward increasing the participation of women in STEM fields. The proposed research promises to enable non-invasive, nanometer-scale microscopy with applications ranging from live-cell imaging to in situ device characterization. The mechanistic understanding of nanoscopic details derived from plasmon-enhanced single-molecule fluorescence will have enormous implications for cell biology, hydrogen storage, chemical separation and energy production.

在化学系化学测量和成像项目的支持下，密歇根大学的朱莉·比廷教授和她的团队正在改进显微镜的功率，以便在1-2纳米尺度上观察活细胞内的蛋白质本身。该项目基于单分子荧光显微镜和金属纳米粒子等离子体动力学的耦合，以前所未有的分辨率和灵活性产生一种非摄动方法，用于观察自然细胞环境中的蛋白质位置和动力学。跨学科的方法旨在(1)了解等离子体增强单分子荧光的机制，(2)在此基础上表征等离子体表面，(3)定位和跟踪与金属纳米颗粒偶联的活细菌细胞中的膜蛋白，以及(4)通过演示，指导，教学和实验室研究让学生接触现代科学，特别着眼于增加女性在STEM领域的参与。该研究有望实现非侵入性纳米级显微镜，应用范围从活细胞成像到原位设备表征。等离子体增强单分子荧光对纳米级细节的机理理解将对细胞生物学、储氢、化学分离和能源生产产生巨大的影响。