Predicting and Controlling the Coupling Between Dyes and Plasmonic Nanoparticles

预测和控制染料与等离子体纳米粒子之间的耦合

• 批准号: 1807676
• 负责人: Julie Biteen
• 金额: $ 39万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807676&HistoricalAwards=false
• 关键词: Predicting; Controlling; Coupling; Between; Dyes

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Biteen at the University of Michigan is developing a microscope to measure position and motion of biomolecules in living cells with a super high resolution (at the nanometer scale). This super-resolution microscope combines emerging high-resolution microscopy techniques and the unique properties metal nanoparticles where metal nanoparticles strongly affect the fluorescence emission from nearby dye molecules. Professor Biteen works on using the high-sensitivity microscopy to understand the interaction between metal nanoparticles and the dye molecules and to control coupled emission. She is working toward a real-time, non-perturbative, biocompatible microscope for live-cell imaging. Professor Biteen works closely with undergraduate women students and students from other under-represented minority groups. She is promoting science as an exciting field to a broad audience by supporting public lectures and developing hands-on demos for middle school girls.Single-molecule, super-resolution microscopy enables direct investigations of physical and optical effects far below the half-micron diffraction limit of light, but this technique is restricted in practical applications by the limited brightness of probes relative to the background, especially in cellular samples. Plasmonic nanoparticles (NPs) readily produce over hundred-fold field intensity enhancements to brighten proximal dyes, but the modified dye emission also yields localization biases that have not yet been fully characterized due to the limitations of conventional experimental approaches. Professor Biteen is improving the resolution of single-molecule imaging with NP plasmonics. State-of-the-art single-molecule fluorescence experiments on dyes coupled to metal NPs can directly measure the effects of plasmonic NPs on proximal dyes. Therefore, the single-molecule readout can inform models of dye-NP interactions, while at the same time, plasmonic enhancement can improve the single-molecule fluorescence brightness. Professor Biteen is working toward high-accuracy, nanometer-scale, plasmon-enhanced super-resolution microscopy by: (1) measuring the changes in dye molecule intensity, spectrum, direction, and polarization of emission produced by a plasmonic NP, (2) controlling the dye-NP coupling by tuning the excitation polarization and frequency according to the relationships determined in objective 1, and (3) super-resolving the positions and movements of membrane proteins in living cells prepared on plasmonic surfaces.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像项目的支持下，密歇根大学的 Biteen 教授正在开发一种显微镜，以超高分辨率（纳米级）测量活细胞中生物分子的位置和运动。这种超分辨率显微镜结合了新兴的高分辨率显微镜技术和独特的金属纳米粒子特性，其中金属纳米粒子强烈影响附近染料分子的荧光发射。 Biteen 教授致力于使用高灵敏度显微镜来了解金属纳米粒子和染料分子之间的相互作用并控制耦合发射。她正在致力于开发一种用于活细胞成像的实时、非扰动、生物相容性显微镜。比特恩教授与本科女学生和来自其他代表性不足的少数群体的学生密切合作。 她通过支持公开讲座和为中学生开发动手演示，向广大观众宣传科学是一个令人兴奋的领域。单分子超分辨率显微镜可以直接研究远低于半微米衍射极限的物理和光学效应，但该技术在实际应用中受到探针相对于背景的有限亮度的限制，尤其是在细胞样本中。等离激元纳米粒子（NP）很容易产生超过一百倍的场强度增强，以增亮邻近的染料，但改进的染料发射也会产生局部偏差，由于传统实验方法的限制，这些偏差尚未完全表征。 Biteen 教授正在利用 NP 等离子体技术提高单分子成像的分辨率。对与金属纳米颗粒偶联的染料进行最先进的单分子荧光实验可以直接测量等离子体纳米颗粒对邻近染料的影响。因此，单分子读数可以为染料-纳米粒子相互作用模型提供信息，同时等离子体增强可以提高单分子荧光亮度。 Biteen 教授正致力于高精度、纳米级、等离子体增强超分辨率显微镜的研究，方法是：(1) 测量等离子体 NP 产生的染料分子强度、光谱、方向和发射偏振的变化，(2) 根据目标 1 中确定的关系，通过调整激发偏振和频率来控制染料-NP 耦合，以及 (3) 超分辨 该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

SMALL-LABS: Measuring Single-Molecule Intensity and Position in Obscuring Backgrounds

• DOI: 10.1016/j.bpj.2019.02.006
• 发表时间: 2019-03-19
• 期刊: BIOPHYSICAL JOURNAL
• 影响因子: 3.4
• 作者: Isaacoff, Benjamin P.;Li, Yilai;Biteen, Julie S.
• 通讯作者: Biteen, Julie S.

Mapping Forbidden Emission to Structure in Self-Assembled Organic Nanoparticles

• DOI: 10.1021/jacs.8b09149
• 发表时间: 2018-11-21
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Hinton, Daniel A.;Ng, James D.;Goldsmith, Randall H.
• 通讯作者: Goldsmith, Randall H.

Model-Based Insight into Single-Molecule Plasmonic Mislocalization

基于模型的单分子等离子体错位洞察

• DOI: 10.1021/acs.jpcc.1c07989
• 发表时间: 2021
• 期刊: The Journal of Physical Chemistry C
• 作者: Zuo, Tiancheng;Goldwyn, Harrison J.;Masiello, David J.;Biteen, Julie S.
• 通讯作者: Biteen, Julie S.

Far-Field Polarization Optics Control the Nanometer-Scale Pattern of High-Fluorescence Dissymmetry Emission from Achiral Molecules near Plasmonic Nanodimers

• DOI: 10.1021/acs.jpcc.3c00467
• 发表时间: 2023-05
• 期刊: The Journal of Physical Chemistry C
• 作者: Zechariah Pfaffenberger;Saaj Chattopadhyay;J. Biteen