CAREER: Microscopic (<100 um) imaging in centimeter-deep tissues based on an ultrasound-switchable fluorescence technique

摘要#1253199 Yuan，Baohong主要研究者建议在厘米深的组织中进行显微成像。他们最近首次开发了一种超声可切换荧光（USF）技术，该技术与当前的成像方法有着根本的不同。在这个项目中，他们将开发一种USF技术，用于深部肿瘤（~2-3 cm）显微成像（100 μ m）。该技术将在组织模体、离体组织样本和活体动物中进行评估和优化。该研究还将在当地和全球范围内与学生，高中教师和公众的教育相结合。这种USF技术将具有高空间分辨率（100 μ m，如高频超声）和相对较大的成像深度（约2-3 cm，如低频超声）。所提出的USF成像技术可以潜在地用于可视化小但厘米深的肿瘤的微观结构、功能和分子信息。它可以大大有利于许多类型的癌症成像，如乳腺癌或前列腺癌。

Abstract#1253199Yuan, BaohongThe PI proposes to conduct microscopic imaging in centimeter deep tissue. They have recently, for the first time, developed an ultrasound-switchable fluorescence (USF) technique that is fundamentally different from current imaging methods. In this project, they will develop a USF technique for deep tumor (~2-3 cm) microscopic imaging (100 ìm). The technique will be evaluated and optimized in tissue phantoms, ex vivo tissue samples, and live animals. The research will also be locally and globally integrated with educations of students, high school teachers and the general public. This USF technique will have high spatial resolution (100 ìm, like high-frequency ultrasound), and relatively large imaging depth (~2-3 cm like low-frequency ultrasound. The proposed USF imaging technique can be potentially used to visualize microscopic structural, functional and molecular information of a small but centimeter deep tumor. It can greatly benefit many types of cancer imaging, such as breast or prostate cancer.