CAREER: Microscopic (<100 um) imaging in centimeter-deep tissues based on an ultrasound-switchable fluorescence technique

职业：微观（

• 批准号: 1253199
• 负责人: Baohong Yuan
• 金额: $ 40.72万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-15 至 2019-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1253199&HistoricalAwards=false
• 关键词: CAREER; Microscopic; &amp; lt; 100

Abstract#1253199Yuan, BaohongThe PI proposes to conduct microscopic imaging in centimeter deep tissue. They have recently, for the first time, developed an ultrasound-switchable fluorescence (USF) technique that is fundamentally different from current imaging methods. In this project, they will develop a USF technique for deep tumor (~2-3 cm) microscopic imaging (100 ìm). The technique will be evaluated and optimized in tissue phantoms, ex vivo tissue samples, and live animals. The research will also be locally and globally integrated with educations of students, high school teachers and the general public. This USF technique will have high spatial resolution (100 ìm, like high-frequency ultrasound), and relatively large imaging depth (~2-3 cm like low-frequency ultrasound. The proposed USF imaging technique can be potentially used to visualize microscopic structural, functional and molecular information of a small but centimeter deep tumor. It can greatly benefit many types of cancer imaging, such as breast or prostate cancer.

摘要#1253199Yuan，Baohong PI提出在厘米深组织中进行显微成像。他们最近首次开发了一种与当前成像方法根本不同的超声可切换荧光（USF）技术。在该项目中，他们将开发一种用于深部肿瘤（~2-3 cm）显微成像（100 µm）的 USF 技术。该技术将在组织模型、离体组织样本和活体动物中进行评估和优化。该研究还将在本地和全球范围内与学生、高中教师和公众的教育相结合。这种 USF 技术将具有较高的空间分辨率（100 µm，如高频超声）和相对较大的成像深度（~2-3 cm，如低频超声）。所提出的 USF 成像技术可用于可视化小但厘米深肿瘤的微观结构、功能和分子信息。它可以极大地有益于多种类型的癌症成像，例如乳腺癌或前列腺癌。