CAREER: A GENERALIZED APPROACH TO THE CHARACTERIZATION OF NATIVE RNA WITHIN THE CELLULAR MILIEU-AN INTEGRATED EDUCATION AND RESEARCH STUDY

1253691-Santangelo这项工作的研究和教育部分的总体重点是细胞生物学和分子工程的整合。在研究部分中，将利用这些学科的整合来开发一种通用的方法，用于在以前发表的单个RNA敏感成像探针的基础上，对细胞环境中的RNA分子进行传感和表征。然后这种方法将被应用于巨核细胞的研究，最近发现巨核细胞可以将RNA分类到血小板形成的位置。在这项提案的教育部分，目标是在本科和中学两级利用积极的学习经验创建教育单元，促进学生将工程学原理和生物学结合起来的能力，并将采用新的评估战略，以衡量其效用。这项建议的目标是在科学层面和社会内部产生广泛的影响。从科学的角度来看，考虑到RNA在几乎所有生物学问题中的作用，允许对其进行彻底表征的新工具将影响许多研究人员的科学。在社会层面上，通过提高学生将工程学方法与细胞生物学相结合的能力，我们将能够提高他们的总体能力，即批判性思维和使用他们在教育期间接触到的所有工具解决问题的能力。

1253691 - SantangeloThe overall focus of the research and education components of this work is on the integration of cell biology and molecular engineering. Within the research component, integration of these disciplines will be utilized in the development of a generalized approach for the sensing and characterization of RNA molecules in the cellular milieu, based on previously published single RNA sensitive imaging probes. This approach will then be applied to the study of megakaryocytes, which recently were discovered to sort RNAs to sites of platelet formation. In the education portion of this proposal, the goal is to create education modules, utilizing active learning experiences, at both the undergraduate and secondary school level, that facilitate the ability of students to integrate engineering principles and biology, and new assessment strategies will be employed in order to gauge their utility. This proposal is targeted to have broad impact at both the scientific level and within society. From a scientific perspective, given the role of RNA in almost every biological problem, new tools that allow for its thorough characterization will impact the science of many researchers. On a societal level, by improving the ability of students to integrate engineering approaches with cellular biology, we?ll be able to improve their general ability to think critically and solve problems using all the tools they have been exposed to during their education.