CAREER: Mapping Regulatory Networks in Extremophiles

职业：绘制嗜极微生物的调控网络

• 批准号: 1254754
• 负责人: Lydia Contreras
• 金额: $ 40.73万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2018-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1254754&HistoricalAwards=false
• 关键词: CAREER; Mapping; Regulatory; Networks; Extremophiles

1254754ContrerasThe PI proposes establishing new approaches for understanding and "reprogramming" RNA-mediated regulatory circuits in vivo. These not well defined networks are essential for the coordination of cellular processes in response to changes in environmental inputs and are vital for survival under high-stress conditions. It is proposed to map and characterize a regulatory network in Zymomonas mobilis using a method developed in the PI's laboratory and to investigate a model regulatory RNA that was found to differentially express based on oxygen levels. The proposed research is scientifically significant because it will provide new fundamental insight into how large-scale molecular networks that involve both RNAs and proteins operate in the context of the cellular machinery. In addition, the PI will focus educational efforts on attracting and retaining underrepresented students to engineering by (i) creating a "Raising Future Scientist" program for students and their families, (ii) developing a new course to present bioengineering in the context of significant world problems, and (iii) continuing commitment to the existing undergraduate research program.

1254754Contreras PI提出建立新的方法来理解和“重编程”RNA介导的体内调控回路。这些未明确定义的网络对于协调细胞过程以应对环境输入的变化至关重要，并且对于在高压力条件下的生存至关重要。建议使用PI实验室开发的方法绘制和表征运动发酵单胞菌中的调控网络，并研究发现基于氧水平差异表达的模型调控RNA。 这项研究具有重要的科学意义，因为它将为涉及RNA和蛋白质的大规模分子网络如何在细胞机制中运作提供新的基本见解。 此外，PI将集中教育努力吸引和留住代表性不足的学生工程（一）创建一个“培养未来的科学家”计划，为学生和他们的家庭，（二）开发一个新的课程，目前生物工程的背景下，重大的世界问题，和（三）继续致力于现有的本科研究计划。