PRODUCTION CENTER FOR MAPPING REGULATORY REGIONS OF THE HUMAN GENOME

摘要 尽管我们确定个体基因组序列的能力变得越来越容易，但我们对 大多数人类基因组的功能是有限的。监管信息的映射尤其是 至关重要，因为大多数(&gt；85%)与人类疾病相关的常见变异位于编码区之外。 ENCODE的前几个阶段为绘制管理要素图作出了重要贡献 但还有更多的工作要完成，包括绘制调控区域的图谱 在更多的组织中，以单个细胞的分辨率以及确定准确的 调节因子，即结合每个区域的转录因子。在我们的斯坦福Encode生产中心 管理区域映射，我们计划使用我们开发的技术来映射绑定 用染色质免疫沉淀法测定五种主要细胞系中大多数转录因子的位置 已标记的TF。我们将通过分析开放的染色质区域来进一步扩展调控元件的目录 在这些细胞系以及来自正常和疾病人类的各种组织和细胞类型中 受试者同意开放获取。最后，我们将定位开放的染色质区域和表达 这些类型的生物样本中的细胞。这些研究将极大地扩大监管区域的目录 人类基因组。将数据快速存入编码数据协调中心(DCC)将 确保快速公开发布。我们预计该中心生成的数据和单元线将是 为广大科学界提供了宝贵的资源。

ABSTRACT Although our ability to determine the genome sequence of individuals is becoming facile, our understanding of the function of most of the human genome is limited. Mapping of regulatory information is particularly crucial since most (>85%) common variants associated with human disease lie outside of coding regions. Previous phases of ENCODE have made important contributions to the mapping of regulatory elements across the genome, but there is much more to be achieved, including the mapping of regulatory regions across many more tissues and at the resolution of individual cells as well as determining the exact regulators i.e. transcription factors that bind each region. In our Stanford ENCODE Production Center for Mapping of Regulatory Regions, we plan to use technologies that we have developed to map the binding sites for most transcription factors (TFs) in five major cell lines using chromatin immunoprecipitation of tagged TFs. We will further expand the catalog of regulatory elements by analyzing open chromatin regions in these cell lines and a wide variety of tissues and cell types from both normal and diseased human subjects consented for open access. Finally, we will map open chromatin regions and expression in single cells from these types of biosamples. These studies will greatly expand the catalog of regulatory regions in the human genome. The rapid deposition of data into the ENCODE Data Coordinating Center (DCC) will ensure quick public release. We expect the data and cell lines that are generated by this Center will be a valuable resource for the broad scientific community.