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Molecular Characterization of Target Scheduling in Bacterial

细菌靶标调度的分子表征

基本信息

批准号：
1716777
负责人：
Lydia Contreras
金额：
$ 54万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2023-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1716777&HistoricalAwards=false
关键词：
Molecular Characterization Target Scheduling Bacterial

项目摘要

In this project, researchers and their students are working to better understand how living organisms respond to their environments. Determining how external stimuli affect how cells perform the metabolic functions that sustain life is of major interest. This project aims to provide basic mechanistic knowledge about how living systems react to maintain normal "healthy" survival patterns while exposed to different external stresses that could lead to unhealthy states. These studies will support the interdisciplinary training of two graduate and three undergraduate students, including undergraduates recruited from a minority serving institution. This project will also support outreach to a low-income, underrepresented community through the existing "Raising Future Scientists" program, where middle school children and their families visit the research laboratory for an introduction to science and engineering, and to science teachers and students at regular "Science and a Movie" events. An important feature of environmental stress responses is that they are mediated by highly dynamic networks. This is particularly true concerning the mechanisms by which global RNA-protein networks regulate multiple metabolic pathways simultaneously post-stress. Given an increasing number of findings that suggest differential target selectivity, this project seeks to identify molecular features that lead to the differential regulation of specific gene subsets at a specific time or after a specific stress. The project will apply a novel approach that employs recently developed in vivo molecular characterization tools and quantitative modeling approaches using the Carbon Storage Regulatory network as a model system to: (i) establish differentially regulated target networks under different stresses, (ii) characterize molecular features of different sets of targets that are differentially regulated by this pathway, and (iii) establish functional relevance of global regulation via formal network analysis. This work focuses on genes that are drastically differentially regulated post-stress by the Csr pathway and will contribute to understanding dynamic networks involved in global metabolic regulation at the molecular level. These studies will have the broader impact of expanding current designs of synthetic gene control schemes and of establishing a general platform for studying the function of global regulators in the context of their entire native networks. This project is funded by the Systems and Synthetic Biology Program in the Division of Molecular and Cellular Biosciences.

在这个项目中，研究人员和他们的学生正在努力更好地了解生物体如何对其环境做出反应。确定外部刺激如何影响细胞如何执行维持生命的代谢功能是人们最感兴趣的。该项目旨在提供有关生命系统在暴露于可能导致不健康状态的不同外部压力时如何反应以维持正常“健康”生存模式的基本机械知识。这些研究将支持两名研究生和三名本科生的跨学科培训，其中包括从少数族裔服务机构招收的本科生。该项目还将通过现有的“培养未来科学家”计划，支持向低收入、代表性不足的社区进行宣传，中学生及其家人可以参观研究实验室，了解科学和工程知识，并定期参加“科学与电影”活动，向科学教师和学生介绍科学和工程。环境应激反应的一个重要特征是它们是由高度动态的网络介导的。对于全球 RNA-蛋白质网络在应激后同时调节多种代谢途径的机制来说尤其如此。鉴于越来越多的研究结果表明目标选择性存在差异，该项目旨在识别导致特定时间或特定应激后特定基因子集差异调节的分子特征。该项目将采用一种新颖的方法，采用最近开发的体内分子表征工具和定量建模方法，使用碳储存调节网络作为模型系统：（i）在不同压力下建立差异调节的目标网络，（ii）表征受该途径差异调节的不同目标组的分子特征，以及（iii）通过正式网络分析建立全球调节的功能相关性。这项工作重点关注在应激后通过 Csr 途径进行显着差异调节的基因，并将有助于在分子水平上理解参与全球代谢调节的动态网络。这些研究将产生更广泛的影响，扩大当前合成基因控制方案的设计，并建立一个通用平台来研究全球监管机构在整个本地网络背景下的功能。该项目由分子和细胞生物科学部的系统和合成生物学计划资助。