Engineering an Efficient Electron Pathway for Photosynthetic Hydrogen Production

设计光合产氢的有效电子途径

• 批准号: 1264289
• 负责人: James Swartz
• 金额: $ 30万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-15 至 2016-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1264289&HistoricalAwards=false
• 关键词: Engineering; Efficient; Electron; Pathway; Photosynthetic

1264289 Swartz, James R.The long-term goal of this program is to engineer self-replicating bacterial systems to efficiently absorb sunlight and use the energy to split water and produce hydrogen. This crucial subproject is designed to provide fundamental insights required for the design of electron transfer pathways generally as well as the engineering and evolution of a specific photosynthetic hydrogen-producing pathway. The project will develop and apply methods that enable the evolution of a fusion protein that docks at photosystem I on a thylakoid membrane. The fusion protein will also be engineered and evolved to efficiently transfer electrons from the photosystem to an [FeFe] hydrogenase. The specific aims include (i)development of a high-throughput in vitro screen for hydrogen production driven by photosynthesis; (ii)engineering of a ferredoxin-hydrogenase fusion enzyme that produces hydrogen by coupling to photosystem I and (iii) the characterization of the productivity and oxygen sensitivity of the engineered photosynthetic hydrogen production system. The broader impacts are significant both for training and technological applications. It will teach collaboration, teamwork and communication skills to a diverse group of students including minorities. The broader application of the principles revealed by this program could reshape the energy and chemical industries and help to provide a sustainable global socio-economic system.

该计划的长期目标是设计自我复制的细菌系统，以有效地吸收阳光，并利用能量分解水并产生氢气。这个重要的子项目的目的是提供电子转移途径的设计所需的基本见解一般以及一个特定的光合产氢途径的工程和进化。 该项目将开发和应用使融合蛋白能够在类囊体膜上停靠在光系统I上的方法。 融合蛋白也将被改造和进化，以有效地将电子从光系统转移到[FeFe]氢化酶。 具体目标包括：（i）开发用于光合作用驱动的氢产生的高通量体外筛选;（ii）通过与光系统I偶联产生氢的铁氧还蛋白-氢化酶融合酶的工程化;以及（iii）工程化的光合氢产生系统的生产率和氧敏感性的表征。更广泛的影响对培训和技术应用都很重要。 它将向包括少数民族在内的各种学生群体传授协作、团队精神和沟通技能。 该计划所揭示的原则的更广泛应用可以重塑能源和化学工业，并有助于提供可持续的全球社会经济体系。