Enabling Highly Sensitive and Specific Detection from Undiluted Blood Using Paper Sensors

使用纸传感器对未稀释的血液进行高度灵敏和特异性的检测

• 批准号: 1264470
• 负责人: Shaoyi Jiang
• 金额: $ 33万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1264470&HistoricalAwards=false
• 关键词: Enabling; Highly; Sensitive; Specific; Detection

Point-of-care devices require simple yet inexpensive platforms with highsensitivity from complex media. Paper is the least expensive platform for assaydevelopment and often allows results to be obtained faster than conventional sensors.Current efforts to improve paper-based detection technologies rely on physicallyadsorbed ligands (with limited ligand amount and activity), rely on blocking agents topassivate nonspecific protein adsorption (with limiting detection sensitivity and suitableonly for simple media or diluted samples), and suffers from low sample retention due tothe rapid evaporation of samples (typically receiving less than 50% of the originalsample in the detection zone). Zwitterionic poly(carboxybetaine) (PCB) surface coatingshave been able to achieve highly sensitive and specific detection from undiluted humanplasma and serum. The objective of this work is to realize inexpensive, yet highlysensitive paper-based detection from complex media using zwitterionic chemistry.

床旁设备需要简单而廉价的平台，对复杂介质具有高灵敏度。纸是最便宜的检测开发平台，通常可以比传统传感器更快地获得结果。目前，改善纸基检测技术的努力依赖于物理吸附的配体（配体量和活性有限），依赖封闭剂钝化非特异性蛋白质吸附（检测灵敏度有限，仅适用于简单介质或稀释样品），并且由于样品的快速蒸发（通常在检测区接收小于50%的原始样品）而遭受低的样品保留。两性离子聚羧基甜菜碱（PCB）表面涂层能够实现对未稀释的人血浆和血清的高灵敏度和特异性检测。这项工作的目的是实现廉价的，但highlysensitive纸为基础的检测从复杂的媒体使用两性离子化学。