I-Corps: Translation of High Frequency Irreversible Electroporation (H-FIRE) for Human Clinical Applications via the Veterinary Oncology Market

高频不可逆电穿孔（H-FIRE）是一种新型的微创非热组织消融技术，将肿瘤暴露在非常短的电场爆发中约2分钟。这些脉冲在结构上重新排列细胞的脂质双分子层，从而诱导细胞死亡。与其他局灶消融技术相反，H-FIRE不影响大血管、神经或细胞外基质，使不可切除的肿瘤可治疗。该项目的目标是将实体肿瘤治疗的基础技术从实验室转移到临床应用。这种微创技术的发展促进了目前无法通过手术切除或其他局灶消融技术进行手术的肿瘤的治疗，因为它们靠近神经和大血管等关键结构。已知某些肿瘤类型具有化疗耐药性，因此必须严格控制辐射暴露，特别是对年轻患者。热烧蚀技术受到局部血管的热沉效应的影响，对处理区域的严格控制是具有挑战性的。H-FIRE作为一种护理标准，有可能补充这些其他技术。如果成功，该项目的直接结果将是开发一种仪器，用于临床医生和研究人员评估基于H-FIRE的疗法。

High Frequency Irreversible Electroporation (H-FIRE) is a new, minimally invasive, non-thermal tissue ablation technique in which a tumor is exposed to bursts of very short electric fields for approximately 2 minutes. These pulses structurally rearrange the lipid bilayer of cells a target region inducing cell death. As opposed to other focal ablation techniques, H-FIRE does not affect major blood vessels, nerves or the extracellular matrix making unresectable tumors treatable. The goal of this project is to transfer fundamental technology for solid tumor treatment from the laboratory to clinical application.Development of this minimally invasive technology facilitates the treatment of tumors currently inoperable with surgical resection or other focal ablation technologies due to their proximity to critical structures such as nerves and major blood vessels. Some tumor types are known to become chemo-resistant and radiation exposure must be closely regulated, especially in younger patients. Thermal-ablation techniques suffer from heat sinking effects by local vessels and tight control of treated regions is challenging. H-FIRE has the potential to complement these other technologies as a standard of care. If successful, a direct result of this project will be the development of an instrument which will be made available for clinicians and researchers to evaluate H-FIRE based therapies.