Bio-inorganic hybrid membranes with nanoporosity control by genetically engineered viral seal rings

通过基因工程病毒密封环控制纳米孔隙度的生物无机杂化膜

• 批准号: 210521111
• 负责人: Dr. Hartmut Gliemann
• 金额: --
• 依托单位: Zentrale Einrichtung Elektronenmikroskopie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/210521111?language=en
• 关键词: Bio; inorganic; hybrid; membranes; nanoporosity

A novel preparation route is proposed to obtain bio/inorganic hybrid membranes with arrays of nanoscaled pores. The procedure allows controlling the distance between pores as well as their diameter. For that purpose, a virus template-assisted solid state membrane approach will be de-veloped. Specifically, Tobacco mosaic virus (TMV) assembly intermediates forming protein disks of precise dimensions with an inner 4 nm pore and an outer diameter of 18 nm will be used. These inter-mediates may be genetically tailored on both, the inner hole and the outer rim surfaces. Hence, combining functionalized inorganic templates (MT) with TMV "pore adapter inlays" might yield mem-branes benefiting from both a stable inorganic backbone and nanopores with near-to-zero size devi-ations. The intended experimental strategy interconnects biogenic and inorganic phases by a bionic „glue‟: peptide-mediated interaction with solid silica and subsequent protein-induced SiO2 deposition should immobilize the TMV disks in MTs and seal gaps. Primary goals are to produce layered membranes with specifically addressable, functionalized conical pores (Ø 15-30 nm) and genetically tailored trifunctional TMV protein disks, to position and pre-fix them into pores by help of intermediate chemical coupling, and to finish the assemblies by rugged biogenic mineral ridges formed between the two material phases. Quality and permeability of the hybrid membrane materials will be analyzed.

提出了一种制备具有纳米孔阵列的生物/无机杂化膜的新方法。该过程允许控制孔之间的距离以及它们的直径。为此目的，将去除病毒模板辅助的固态膜方法。具体地，将使用烟草花叶病毒（TMV）组装中间体，其形成具有4 nm内孔和18 nm外径的精确尺寸的蛋白质盘。这些中间体可以在内孔和外缘表面两者上进行基因定制。因此，将功能化无机模板（MT）与TMV“孔适配器嵌体”组合可能产生受益于稳定的无机骨架和具有接近零尺寸偏差的纳米孔的膜。预期的实验策略通过仿生“胶水”将生物相和无机相互连：肽介导的与固体二氧化硅的相互作用和随后的蛋白质诱导的SiO2沉积应将TMV盘包裹在MT中并密封间隙。主要目标是生产具有特定可寻址的、功能化的锥形孔（约15-30 nm）和遗传定制的三功能TMV蛋白盘的分层膜，以通过中间化学偶联的帮助将它们定位和预先固定到孔中，并通过在两个材料相之间形成的粗糙的生物矿物质脊来完成组装。将分析杂化膜材料的质量和渗透性。