Central metabolism of Archaea

古细菌的中枢代谢

• 批准号: 210757926
• 负责人: Professor Dr. Ivan A. Berg
• 金额: --
• 依托单位: Institut für Molekulare Mikrobiologie und Biotechnologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/210757926?language=en
• 关键词: Central; metabolism; Archaea

The Archaea form the third domain of life and possess many genetical, structural and biochemical features which are considered to be ancestral. An interesting feature of Archaea is a great diversity of their central metabolic pathways, many of which have never been identified in either Bacteria or Eukarya. In the last years, we have identified two novel autotrophic CO2 assimilation pathways in Crenarchaeota and an unusual acetate assimilation pathway in haloarchaea. Although these and other studies substantially increased our understanding of the archaeal physiology and biochemistry, they also identified several gaps in our knowledge of archaeal central metabolic pathways. Such gaps are: (i) the gene encoding 4-hydroxybutyrate-CoA ligase in Metallosphaera sedula is not known and should be identified and characterized; (ii) the pathway of autotrophic CO2 fixation in mesophilic marine group I Archaea (‘Thaumarchaeota’) remains to be proven biochemically; (iii) the pathway of autotrophic CO2 fixation in some Pyrobaculum species is unknown; (iv) the methylaspartate cycle of acetyl-CoA assimilation in Haloarcula marismortui requires further experimental support as well as identification and characterization of its key enzymes. These questions will be addressed experimentally using different microbiological, biochemical and genetical approaches.

古生物是生命的第三个领域，具有许多遗传、结构和生物化学特征，被认为是祖先的。一个有趣的特征是它们的中央代谢途径的多样性，其中许多从未在细菌或真核生物中被发现。在过去的几年里，我们已经确定了两个新的自养CO2同化途径Crenarchaeota和一个不寻常的乙酸盐同化途径在盐古菌。虽然这些和其他研究大大增加了我们对古细菌生理学和生物化学的理解，但它们也确定了我们对古细菌中心代谢途径的认识中的几个空白。这些差距是：（i）不知道在Medallosphaera sedula中编码4-羟基丁酸-CoA连接酶的基因，应当对其进行鉴定和表征;（ii）嗜温海洋第I组水母中的自养CO2固定途径（iii）某些火杆菌属物种的自养CO2固定途径尚不清楚;（iv）海盐蚌乙酰辅酶A同化的甲基天冬氨酸循环需要进一步的实验支持以及其关键酶的鉴定和表征。这些问题将使用不同的微生物，生物化学和遗传学方法进行实验。