Collaborative Research: Development of Novel Nonlinear Optical Contrast for High-Resolution Morphological and Chemical Imaging of Historical Artwork

在化学部的化学测量和成像计划以及生物基础设施部（DBI）的生物研究仪器开发（IDBR）计划的支持下，Martin Fischer教授，Warren Warren，Robert Opila，Jennifer Mass博士及其团队将开发一种用于无损识别和高分辨率的新型成像方法，三维映射的组织和广泛的色素样品的历史艺术作品。拟议的研究的主要重点将是建立一个多模态非线性光学显微镜的非侵入性，高分辨率成像并使用脉冲整形和时间-同时获得吸收和折射对比度的解析检测技术，并进一步开发用于组织的新型分析和表征方法样品和颜料。然后，这些小组将建立厚组织、油漆横截面和完整历史绘画的三维（3D）成像，并进一步增加分子吸收和非线性相位对比的成像深度。研究人员将开发一种便携式显微镜，用于现场应用，然后将通过工业合作伙伴传播所开发的核心技术。拟议的研究如果成功，将能够在保护环境中进行原位应用，以避免不得不将艺术品从其气候控制环境中移除，这将通过提供一种非侵入性成像方式来极大地影响保护科学，该成像方式可以从历史艺术品中提取全面的结构和化学信息，补充已建立的绘图技术。 开发的技术还将为广泛的生物界提供工具，以检查生物组织中的结构，代谢和功能对比。该计划将为研究生，本科生和高中生提供跨学科环境中的培训机会，涵盖保护科学，艺术史，物理，化学，生物和工程。

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry and the Instrument Development for Biological Research (IDBR) program in the Division of Biological Infrastructure (DBI), Professors Martin Fischer, Warren Warren, Robert Opila, Dr. Jennifer Mass and their groups will develop a novel imaging method for nondestructive identification and high-resolution, three-dimensional mapping of both tissue and a broad range of pigment samples in historic works of art. The primary focus of the proposed research will be to set up a multimodal nonlinear optical microscope for noninvasive, high-resolution imaging and use pulse shaping and time-resolved detection technology for simultaneous access to absorptive and refractive contrasts and further develop novel analysis and characterization methods for tissue samples and pigments. The groups will then set up three-dimensional (3D) imaging of thick tissue, paint cross-sections and intact historic paintings and further increase imaging depth for molecular absorption and nonlinear phase contrast. The researchers will develop a portable microscope for applications in the field and the core technology developed will then be disseminated through industrial partners.The proposed research, if successful, will enable in situ applications in a conservation setting to avoid having to remove artworks from their climate-controlled environment, which will dramatically impact conservation science by providing a non-invasive imaging modality that can extract comprehensive structural and chemical information from historic artwork, complementing established mapping techniques. The technology developed will also provide tools to the broad biology community to examine structural, metabolic and functional contrast in biological tissue. The program will provide training opportunities in an interdisciplinary environment spanning conservation science, art history, physics, chemistry, biology, and engineering for graduate, undergraduate, and high-school students.