Collaborative Research: Development of Novel Nonlinear Optical Contrast for High-Resolution Morphological and Chemical Imaging of Historical Artwork

在化学部门的化学测量和成像项目以及生物基础设施部门的生物研究仪器开发（IDBR）项目的支持下，Martin Fischer教授、Warren Warren、Robert Opila、Jennifer Mass博士和他们的团队将开发一种用于无损鉴定和高分辨率的新型成像方法。历史艺术作品中组织和广泛的颜料样本的三维绘图。该研究的主要重点将是建立一个多模态非线性光学显微镜进行无创、高分辨率成像，并使用脉冲整形和时间分辨检测技术同时获得吸收和折射对比，并进一步开发新的组织样品和色素分析和表征方法。然后，研究小组将建立厚组织的三维（3D）成像，绘制横截面和完整的历史绘画，并进一步增加分子吸收和非线性相位对比的成像深度。研究人员将开发一种用于该领域的便携式显微镜，然后开发的核心技术将通过工业伙伴传播。这项研究如果成功，将使保护环境中的原位应用成为可能，从而避免将艺术品从气候控制的环境中移走，这将极大地影响保护科学，因为它提供了一种非侵入性的成像方式，可以从历史艺术品中提取全面的结构和化学信息，补充了现有的制图技术。所开发的技术还将为广泛的生物界提供工具，以检查生物组织中的结构，代谢和功能对比。该项目将为研究生、本科生和高中生提供跨学科环境下的培训机会，涵盖保护科学、艺术史、物理学、化学、生物学和工程学。

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry and the Instrument Development for Biological Research (IDBR) program in the Division of Biological Infrastructure (DBI), Professors Martin Fischer, Warren Warren, Robert Opila, Dr. Jennifer Mass and their groups will develop a novel imaging method for nondestructive identification and high-resolution, three-dimensional mapping of both tissue and a broad range of pigment samples in historic works of art. The primary focus of the proposed research will be to set up a multimodal nonlinear optical microscope for noninvasive, high-resolution imaging and use pulse shaping and time-resolved detection technology for simultaneous access to absorptive and refractive contrasts and further develop novel analysis and characterization methods for tissue samples and pigments. The groups will then set up three-dimensional (3D) imaging of thick tissue, paint cross-sections and intact historic paintings and further increase imaging depth for molecular absorption and nonlinear phase contrast. The researchers will develop a portable microscope for applications in the field and the core technology developed will then be disseminated through industrial partners.The proposed research, if successful, will enable in situ applications in a conservation setting to avoid having to remove artworks from their climate-controlled environment, which will dramatically impact conservation science by providing a non-invasive imaging modality that can extract comprehensive structural and chemical information from historic artwork, complementing established mapping techniques. The technology developed will also provide tools to the broad biology community to examine structural, metabolic and functional contrast in biological tissue. The program will provide training opportunities in an interdisciplinary environment spanning conservation science, art history, physics, chemistry, biology, and engineering for graduate, undergraduate, and high-school students.