Protein-based Materials with Tunable Adhesive and Mechanical Properties

ID：MPS/DMR/BMAT（7623）1309787 PI：Liu，Julie ORG：Purdue UniversityTitle：具有可调粘合剂和机械性能的蛋白质基材料智能优点：主要的侵入性外科手术会因使用缝线和斯台普斯而引起组织损伤。 设计有效的外科胶水将是变革性的，因为减少了这些传统手术方法造成的组织损伤。 然而，此时，没有合适的材料满足软组织的有效外科粘合剂的所有主要标准：（1）在潮湿环境中固化，（2）产生强粘合，（3）是生物相容的，以及（4）是柔性的以匹配被粘合在一起的软组织的刚度。 该提案将开发新的生物材料，该材料联合收割机了贻贝粘附蛋白的粘附特性，贻贝使用该蛋白质将自身牢固地结合到表面上，以及蛋白质弹性蛋白的柔性特性，这有助于血管承受脉动流的机械需求的能力。 这些研究将建立材料设计参数，调节所提出的生物材料的粘合和机械性能，从而使我们能够为需要外科粘合剂的几种手术定制材料。 具体目标是：（1）开发具有粘附和柔性区域的重组蛋白质家族，（2）建立蛋白质设计与粘附和弹性体性质之间的关系，以及（3）评估所提出的生物材料在生物应用中的潜力。 作为这项工作的结果，我们希望开发新的材料，并证明软组织手术粘合剂的潜力。更广泛的影响：通过提供替代缝线和斯台普斯，拟议的生物材料将改善接受侵入性手术的患者的临床结果。 此外，该生物材料还可以具有其他临床应用，例如植入物涂层和组织工程基质。 拟议的研究将为高中，本科和研究生的培训和指导提供基础。 两位研究人员都致力于激励和鼓励女性和代表性不足的少数民族成为工程师，这一点可以从过去和未来的活动中得到证明。 具体而言，PI将继续与普渡大学女性工程项目合作，并开发一个实验室模块，让K-12女学生参与开发和评估粘合剂材料的概念。 共同主要研究者将继续通过媒体介绍开展公众外联工作。 此外，这项建议将支持社区学院学生积极参与研究。 最后，我们将通过发表期刊文章，在全国会议上介绍，并列入课堂材料传播研究成果。

ID: MPS/DMR/BMAT(7623) 1309787 PI: Liu, Julie ORG: Purdue UniversityTitle: Protein-based Materials with Tunable Adhesive and Mechanical Properties INTELLECTUAL MERIT: Major, invasive surgical procedures introduce tissue damage from the use of sutures and staples. Designing effective surgical glues will be transformative owing to decreased tissue damage that occurs from these traditional joinery methods. At this time, however, there are no suitable materials that fulfill all of the major criteria for an effective surgical adhesive of soft tissues: (1) set in a wet environment, (2) create strong bonds, (3) be biocompatible, and (4) be flexible to match the stiffness of soft tissues being bonded together. This proposal will develop new biomaterials that combine the adhesive properties of mussel adhesive proteins, which mussels use to strongly bind themselves to surfaces, and the flexible properties of the protein elastin, which contributes to the ability of blood vessels to withstand the mechanical demands of pulsatile flow. These studies will establish the material design parameters that modulate the adhesive and mechanical properties of the proposed biomaterials, thus allowing us to tailor the material for several procedures in which surgical adhesives are needed. The specific objectives are to: (1) Develop a family of recombinant proteins with both adhesive and flexible regions, (2) Establish the relationship between protein design and the adhesive and elastomeric properties, and (3) Evaluate the potential of the proposed biomaterials in biological applications. As a result of this work, we expect to develop novel materials and demonstrate the potential for a soft tissue surgical adhesive.BROADER IMPACTS: By providing an alternative to sutures and staples, the proposed biomaterials will improve clinical outcomes for patients undergoing invasive surgeries. Moreover, the biomaterials may have other clinical applications such as implant coatings and tissue-engineered matrices. The proposed research will serve to provide the basis for training and mentoring of high school, undergraduate, and graduate students. Both investigators are committed to motivate and encourage females and underrepresented minorities to become engineers, as evidenced by past and future activities. Specifically, the PI will continue collaborating with the Purdue Women in Engineering Program and develop a laboratory module to engage K-12 female students with the concept of developing and evaluating adhesive materials. The co-PI will continue general public outreach efforts with media presentations. In addition, this proposal will support the active participation of community college students in research. Finally, we will disseminate the research results through published journal articles, presentation at national conferences, and inclusion in classroom material.