Protein-based Materials with Tunable Adhesive and Mechanical Properties

ID：MPS/dmr/bmat(7623)1309787 PI：Liu，Julie ORG：普渡大学题：基于蛋白质的材料具有可调节的粘附性和机械性能智力优点：主要的侵入性外科手术会因使用缝合线和订书机而导致组织损伤。设计有效的手术胶将是变革性的，因为这些传统的细木工方法减少了组织损伤。然而，目前还没有合适的材料来满足有效的软组织外科粘合剂的所有主要标准：(1)在潮湿的环境中粘合，(2)产生牢固的粘合力，(3)生物相容性，(4)柔韧性，以匹配被粘合在一起的软组织的硬度。这项提议将开发新的生物材料，将贻贝粘附性蛋白的粘附性和蛋白质弹性蛋白的弹性特性结合在一起，贻贝粘附性蛋白用于将自己牢固地结合到表面上，弹性蛋白有助于血管承受脉动流的机械要求。这些研究将建立材料设计参数，以调节建议的生物材料的粘附性和机械性能，从而使我们能够为需要手术粘合剂的几种程序量身定做材料。具体目标是：(1)开发具有粘附区和柔性区的重组蛋白质家族，(2)建立蛋白质设计与粘附性和弹性特性之间的关系，以及(3)评估所建议的生物材料在生物应用中的潜力。作为这项工作的结果，我们希望开发新的材料并展示软组织手术粘合剂的潜力。BROADER影响：通过提供缝合线和钉子的替代方案，建议的生物材料将改善接受侵入性手术的患者的临床结果。此外，生物材料还可能有其他临床应用，如植入涂层和组织工程基质。拟议的研究将为高中生、本科生和研究生的培训和指导提供基础。这两位调查人员都致力于激励和鼓励女性和代表性不足的少数族裔成为工程师，过去和未来的活动都证明了这一点。具体地说，PI将继续与普渡女性工程项目合作，并开发一个实验室模块，让K-12女性学生参与开发和评估粘合材料的概念。共同宣传部将通过媒体介绍继续开展一般公众宣传活动。此外，这项建议还将支持社区大学生积极参与研究。最后，我们将通过发表期刊文章、在国家会议上发表报告以及纳入课堂材料来传播研究成果。

ID: MPS/DMR/BMAT(7623) 1309787 PI: Liu, Julie ORG: Purdue UniversityTitle: Protein-based Materials with Tunable Adhesive and Mechanical Properties INTELLECTUAL MERIT: Major, invasive surgical procedures introduce tissue damage from the use of sutures and staples. Designing effective surgical glues will be transformative owing to decreased tissue damage that occurs from these traditional joinery methods. At this time, however, there are no suitable materials that fulfill all of the major criteria for an effective surgical adhesive of soft tissues: (1) set in a wet environment, (2) create strong bonds, (3) be biocompatible, and (4) be flexible to match the stiffness of soft tissues being bonded together. This proposal will develop new biomaterials that combine the adhesive properties of mussel adhesive proteins, which mussels use to strongly bind themselves to surfaces, and the flexible properties of the protein elastin, which contributes to the ability of blood vessels to withstand the mechanical demands of pulsatile flow. These studies will establish the material design parameters that modulate the adhesive and mechanical properties of the proposed biomaterials, thus allowing us to tailor the material for several procedures in which surgical adhesives are needed. The specific objectives are to: (1) Develop a family of recombinant proteins with both adhesive and flexible regions, (2) Establish the relationship between protein design and the adhesive and elastomeric properties, and (3) Evaluate the potential of the proposed biomaterials in biological applications. As a result of this work, we expect to develop novel materials and demonstrate the potential for a soft tissue surgical adhesive.BROADER IMPACTS: By providing an alternative to sutures and staples, the proposed biomaterials will improve clinical outcomes for patients undergoing invasive surgeries. Moreover, the biomaterials may have other clinical applications such as implant coatings and tissue-engineered matrices. The proposed research will serve to provide the basis for training and mentoring of high school, undergraduate, and graduate students. Both investigators are committed to motivate and encourage females and underrepresented minorities to become engineers, as evidenced by past and future activities. Specifically, the PI will continue collaborating with the Purdue Women in Engineering Program and develop a laboratory module to engage K-12 female students with the concept of developing and evaluating adhesive materials. The co-PI will continue general public outreach efforts with media presentations. In addition, this proposal will support the active participation of community college students in research. Finally, we will disseminate the research results through published journal articles, presentation at national conferences, and inclusion in classroom material.