DISSERTATION RESEARCH: Using Anchored Phylogenomics to Test the Validity of Suture Zones and Refugia Through Comparative Phylogeography of Amphibians

论文研究：利用锚定系统基因组学通过两栖动物的比较系统发育地理学来测试缝合带和庇护所的有效性

• 批准号: 1311144
• 负责人: Emily Lemmon
• 金额: $ 1.91万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1311144&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Using; Anchored; Phylogenomics

The processes that promote species diversity are of central importance in evolutionary biology. Suture zones are defined as geographic regions of overlap between multiple pairs of species or lineages that may hybridize in the zone. These areas are of great interest as natural laboratories to study speciation. The leading hypothesis explaining the formation of suture zones is that past changes in climate caused lineages to be isolated in refugia and subsequently expand into areas of overlap. It is unclear, however, whether suture zones are a general phenomenon across all species. This project will combine recent advances in sequencing technology and geographic information systems to: (1) test whether predicted patterns of suture zones exist across multiple frog species in the Southeastern U.S., and (2) test the leading hypothesis explaining their formation. This study will apply a novel method of data collection, anchored phylogenomics, to sequence hundreds of genetic markers across four co-distributed species. This will develop the foundation for future studies to build on a comparative dataset by including the same genomic regions for additional species, providing a powerful way to test the factors underlying spatial genetic variation. The project provides opportunities to promote training of graduate and undergraduate students, assist state wildlife agencies with monitoring amphibian populations, and contribute knowledge to issues of societal concern such as the organismal response to climate change.

促进物种多样性的过程在进化生物学中至关重要。 缝合带被定义为可能在该区域杂交的多对物种或谱系之间重叠的地理区域。 这些地区作为研究物种形成的天然实验室具有很大的意义。 解释缝合带形成的主要假设是，过去气候的变化导致谱系在避难所中被隔离，随后扩展到重叠区域。 然而，尚不清楚缝合带是否是所有物种的普遍现象。 该项目将联合收割机结合测序技术和地理信息系统的最新进展，以：（1）测试在美国东南部的多个青蛙物种中是否存在缝合带的预测模式，（2）检验解释其形成的主要假设。 这项研究将应用一种新的数据收集方法，锚定基因组学，对四个共同分布的物种的数百个遗传标记进行测序。 这将为未来的研究奠定基础，通过包括其他物种的相同基因组区域来建立一个比较数据集，为测试空间遗传变异的潜在因素提供一种强有力的方法。 该项目提供了机会，促进研究生和本科生的培训，协助国家野生动物机构监测两栖动物种群，并为社会关注的问题，如生物对气候变化的反应贡献知识。