Dissertation Research: Turning Vines Into Trees: A Genome Skimming Approach to the Phylogenetics of New World Milkweed VInes (Matelea, subg. Chthamalia, Apocynaceae)

论文研究：将藤蔓变成树木：新世界乳草藤蔓（Matelea，subg. Chthamalia，夹竹桃科）系统发育的基因组撇取方法

• 批准号: 1311170
• 负责人: Mark Fishbein
• 金额: $ 1.89万
• 依托单位: Oklahoma State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1311170&HistoricalAwards=false
• 关键词: Dissertation; Research; Turning; Vines; Into

Although plant adaptations to aridity have been well studied in a few groups, such as cacti, relatively little is known about the diversification of most plants in deserts and other drylands. A group of milkweeds (Matelea subg. Chthamalia) inhabits dry or seasonally dry habitats in Mexico and the southwestern United States, providing a useful system for investigating adaptive diversification in arid bioregions. This group is part of a large, mostly tropical clade of milkweed vines (Gonolobinae) and most exhibit apparent adaptations to aridity. Many have lost their ability to twine (with stems spreading flat on the ground), have small, bell-shaped flowers, or are covered by an abundance of hairs. This research will use the most powerful available techniques to reconstruct the evolutionary history of Chthamalia and to analyze the evolution of adaptations to aridity. Next generation DNA sequencing employing a ?genome skimming? approach will be used to obtain complete chloroplast genomes, partial mitochondrial genomes, and complete nuclear ribosomal gene sequences to robustly estimate phylogenetic relationships, which will serve as a framework for evolutionary analyses of morphological adaptations.This project will resolve the classification of little-known plants that have been taxonomically controversial. Several species of Chthamalia are exceedingly rare; resolving taxonomic questions and greater knowledge of their evolution will contribute to future conservation efforts of species and habitats. Integration of traditional systematic approaches with new molecular methods is critical to address hypotheses about evolution, adaptation, and speciation. Graduate and undergraduate students will gain laboratory and bioinformatic training as well as the opportunity to present research results at national meetings.

尽管植物对干旱的适应性已经在几组中进行了很好的研究，例如仙人掌，但对沙漠和其他旱地中大多数植物的多样化的了解相对较少。一组乳草（MateleaSubg。Chthamalia）居住在墨西哥和美国西南部的干燥或季节性干燥的栖息地，为研究干旱生物园的适应性多样化提供了有用的系统。这一组是大型，主要是米尔毛藤（Gonolobinae）的大型热带进化枝的一部分，并且大多数表现出对干燥的明显适应性。许多人失去了缠绕的能力（茎在地面上张开），有小的钟形花朵，或者被丰富的头发覆盖。这项研究将利用最强大的技术来重建chthamalia的进化历史，并分析适应对干旱的演变。下一代DNA测序采用了基因组略读？方法将用于获得完整的叶绿体基因组，部分线粒体基因组和完整的核核糖体基因序列，以稳健地估计系统发育关系，这将作为形态适应的进化分析的框架。这些项目将解决众所周知的植物的分类，这些植物具有分类为一种具有分类法的典型植物。几种chthamalia种非常罕见。解决分类学问题和对其进化的更多了解将有助于物种和栖息地的未来保护工作。将传统系统方法与新分子方法的整合对于解决有关进化，适应和物种形成的假设至关重要。研究生和本科生将获得实验室和生物信息学培训，以及在国家会议上提出研究结果的机会。