Mechanistic Studies on Quinoline and Other Photoremovable Protecting Groups for Biological Applications

喹啉和其他光可去除保护基团的生物应用机理研究

• 批准号: 1317760
• 负责人: Timothy Dore
• 金额: $ 12.26万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-23 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1317760&HistoricalAwards=false
• 关键词: Mechanistic; Studies; Quinoline; Other; Photoremovable

The Chemical Structure, Dynamics and Mechanisms Program supports Professor Timothy M. Dore at the University of Georgia who will explore the mechanism of photodeprotection and optimize the sensitivity of photoremovable protecting groups (PPG) that are sensitive to two-photon excitation (2PE). A host of spectroscopic techniques will be used to study quinoline and other PPG with sensitivity to 2PE. These materials have the potential to be used to photoregulate the action of biological effectors. Optimizing their photochemical properties for biological applications requires a good understanding of the photodeprotection reaction mechanism. The study will expand the base of photoprotecting groups available, as well as carry out photophysical and photochemical studies aimed at providing an improved understanding of the mechanism by which the systems operate. The research in this proposal will enable the development of novel tools and techniques to apply to fundamental scientific questions in neuroscience, developmental biology, biochemistry, physiology, and medicinal chemistry.With the support of the Chemical Structure, Dynamics and Mechanisms Program in the Chemistry Division at the National Science Foundation, Dr. Timothy M. Dore will prepare appropriate chemical compounds and understand the photophysical characteristics that will make them useful in two-photon excitation for precise 3-D control of biological processes. In addition to the broader impact of the relationship of the work to the biological sciences, the inclusion of undergraduate researchers is a major strength of the proposal. The multidisciplinary nature of this research provides an excellent medium to integrate research and education and to train students to become outstanding scholars with a depth of understanding of organic chemistry and photochemistry. Undergraduate students, including those from underrepresented groups, play a major role in the research. The PI has trained over 25 undergraduates and many are coauthors on articles and manuscripts and have matriculated into doctoral programs. The research plan includes the participation of underrepresented groups in science.

化学结构，动力学和机制计划支持教授蒂莫西M。Dore博士将探索光脱保护的机制，并优化对双光子激发（2PE）敏感的光可去除保护基团（PPG）的灵敏度。许多光谱技术将用于研究喹啉和其他PPG与2PE的敏感性。这些材料具有用于光调节生物效应器的作用的潜力。优化其光化学性质的生物应用需要一个很好的理解的光脱保护反应机制。这项研究将扩大现有的光保护基团的基础，并开展生物物理学和光化学研究，旨在更好地了解系统运作的机制。这项研究将使新的工具和技术的发展，适用于神经科学，发育生物学，生物化学，生理学和药物化学的基础科学问题。多尔将准备适当的化合物，并了解生物物理特性，这将使他们在双光子激发生物过程的精确三维控制有用。除了这项工作与生物科学的关系所产生的更广泛的影响外，本科生研究人员的加入也是这项提议的一个主要优势。这项研究的多学科性质提供了一个很好的媒介，整合研究和教育，培养学生成为优秀的学者与有机化学和光化学的深入了解。本科生，包括那些来自代表性不足的群体，在研究中发挥着重要作用。PI已经培训了超过25名本科生，其中许多人是文章和手稿的合著者，并已被录取进入博士课程。该研究计划包括代表性不足的群体参与科学。