Protein-mRNA Interactions in the Control of Central Carbon Metabolism in Bacillus subtilis

枯草芽孢杆菌中心碳代谢控制中的蛋白质-mRNA 相互作用

• 批准号: 21172300
• 负责人: Professor Dr. Jörg Stülke
• 金额: --
• 依托单位: Abteilung für Allgemeine Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/21172300?language=en
• 关键词: Protein; mRNA; Interactions; Control; Central

Regulation of metabolism has so far mainly been studied at the levels of gene regulation by DNA-binding transcription factors and enzymatic activity control. However, glycolysis in Bacillus subtilis is also regulated by distinct protein-RNA interactions that control either mRNA processing, stability and differential expression of proteins encoded in a single operon or transcription elongation via a termination/antitermination mechanism. We intend to study mRNA processing of the B. subtilis gapA operon encoding the enzymes for triose phosphate interconversions in glycolysis. We plan to study the role of the two newly discovered RNases J in the processing of the gapA operon mRNA. The first step in glycolysis, glucose transport and phophorylation, is controlled by a protein-dependent RNA switch in B. subtilis. We want to understand the molecular details of the reconnaissance between the protein, GlcT, and its RNA target sequence. This will be addressed by crystallographic and genetic approaches. Moreover, we wish to study the intramolecular signal transduction process in GlcT that links glucose availability and RNA binding activity to the expression or not of the ptsGHI operon.

迄今为止，代谢的调节主要在通过DNA结合转录因子的基因调节和酶活性控制的水平上进行研究。然而，枯草芽孢杆菌中的糖酵解也受到不同的蛋白质-RNA相互作用的调节，所述相互作用控制在单个操纵子中编码的蛋白质的mRNA加工、稳定性和差异表达或通过终止/抗终止机制的转录延伸。本实验旨在研究B的mRNA加工。枯草芽孢杆菌gapA操纵子，编码糖酵解中磷酸丙糖相互转化的酶。我们计划研究两个新发现的RNases J在gapA操纵子mRNA加工中的作用。糖酵解的第一步，葡萄糖转运和磷酸化，由B中的蛋白质依赖性RNA开关控制。枯草杆菌。我们希望了解蛋白质GlcT及其RNA靶序列之间侦察的分子细节。这将通过晶体学和遗传学方法来解决。此外，我们希望研究在GlcT的分子内信号转导过程，链接葡萄糖的可用性和RNA结合活性的表达或不的ptsGHI操纵子。