Riboswitches and small RNAs in Bacilli under different thermal conditions

不同温度条件下杆菌中的核糖开关和小RNA

• 批准号: 39952618
• 负责人: Professor Dr. Jörg Stülke
• 金额: --
• 依托单位: Abteilung für Allgemeine Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39952618?language=en
• 关键词: Riboswitches; small; RNAs; Bacilli; under

Bacteria that grow at different temperatures have to adapt to these conditions. This has intensively been studied for proteins and lipids. However, with the recent discovery of the general importance of small RNAs and riboswitches in the control of gene expression, metabolism and physiology in bacteria, it seems obvious that the novel RNA elements must be optimized to work at the temperature their organism lives at. The bacilli are a group of Gram-positive bacteria that have a representative (B. subtilis) that belongs to the best-understood organisms. On the other hand, many members of this group live under rather harsh conditions, among them low and high temperatures. We will therefore identify novel small RNA regulatory elements with a special emphasis on carbon storage regulation and the psychrophilic and thermophilic organisms. For some of these elements, we will identify the biological function. With these information at hand, we will then initiate a study on the activity of the regulatory elements at different temperatures. Based on this study, we will perform a mutagenesis to identify the determinants that are responsible for the adaptation of the RNA regulators to different temperatures.

在不同温度下生长的细菌必须适应这些条件。这已被深入研究的蛋白质和脂质。然而，随着最近发现小RNA和核糖开关在细菌基因表达、代谢和生理学控制中的普遍重要性，似乎很明显，新的RNA元件必须优化以在其生物体生活的温度下工作。杆菌是一组革兰氏阳性菌，具有代表性的（B.枯草杆菌），属于最了解的生物体。另一方面，这一群体的许多成员生活在相当恶劣的条件下，其中包括低温和高温。因此，我们将确定新的小RNA调控元件，特别强调碳储存调节和嗜冷和嗜热生物。对于其中一些元素，我们将确定其生物功能。有了这些信息，我们将开始研究调节元件在不同温度下的活性。基于这项研究，我们将进行诱变，以确定负责RNA调节因子适应不同温度的决定因素。