I-Corps: Probiotics to Prevent Metabolic Changes Associated with Starch Induced Laminitis

I-Corps：益生菌可预防与淀粉引起的蹄叶炎相关的代谢变化

• 批准号: 1342640
• 负责人: Jeffrey Blanchard
• 金额: $ 5万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1342640&HistoricalAwards=false
• 关键词: Corps; Probiotics; Prevent; Metabolic; Changes

Through the use of genetic tools, researchers have identified naturally occurring bacteria that appear to consume lactic acid thus maintaining gastro-intestinal balance in animals under high starch conditions. These bacteria may provide the "right" microbial participants to prevent or minimize the extent of laminitis in horses and ruminant livestock. Preliminary data verifies that the in vitro system models fluctuations in bacterial metabolites seen in the horse gut during starch-induced laminitis and enables the tracking of bacterial populations over time. One group of bacteria, the Veillonellaceae, show promise as a probiotic for the ability to utilize lactic acid, which is known to increase during starch induction, and thought to be implicated in the metabolic cascade leading to laminitis. The research team has isolated strains of Veillonellaceae with potential to utilize lactate and withstand low pH and storage conditions. This proposal supports further work to: a) use the in vitro system to demonstrate that isolated strains, individually and in mixtures, can prevent or ameliorate microbial changes characteristic of starch induced acidosis (laminitis) in horse and cattle GI samples; b) conduct dose titration studies to determine effective concentrations of strains or mixtures and; c) determine the robustness of effective strains, namely growth rate on selective media, oxygen tolerance, and lyophilization stability (desiccation tolerance). The anticipated outcome for this project is a prototype formulation ready for testing on either an animal model of induced lactic acidosis or a cohort of volunteer clinical cases with chronic pasture laminitis.A new understanding of the microbial dynamics underlying lactic acidosis and laminitis may lead to better informed models of health, improved feed formulations and management practices, and the development of a probiotic treatment for affected horses that can be used to prevent and/or treat a condition affecting millions of horses annually. These bacteria can be incorporated into animal feed to protect from the fermentative events that trigger laminitis and this could potentially help improve beef and dairy production by decreasing the number of cases of laminitis among cattle, as well as reducing medical costs for horse owners.

通过使用遗传工具，研究人员已经确定了天然存在的细菌，这些细菌似乎消耗乳酸，从而在高淀粉条件下维持动物的胃肠平衡。这些细菌可以提供“正确的”微生物参与者，以预防或最大限度地减少马和反刍家畜的蹄叶炎。初步数据证实，体外系统模拟了淀粉诱导的蹄叶炎期间马肠道中细菌代谢物的波动，并能够随着时间的推移跟踪细菌种群。一组细菌，韦荣氏球菌科，显示出作为益生菌利用乳酸的能力的希望，已知乳酸在淀粉诱导期间增加，并且被认为与导致蹄叶炎的代谢级联有关。研究小组已经分离出Veillonellaceae菌株，这些菌株具有利用乳酸盐的潜力，并能承受低pH值和储存条件。该建议支持进一步的工作：a）使用体外系统证明分离的菌株，单独或混合，可以预防或改善淀粉诱导的酸中毒的微生物变化特征B）进行剂量滴定研究以确定菌株或混合物的有效浓度; c）确定有效菌株的稳健性，即在选择性培养基上的生长速率、氧耐受性和冻干稳定性（干燥耐受性）。该项目的预期成果是一种原型配方，可用于诱导性乳酸酸中毒的动物模型或慢性牧场蹄叶炎志愿者临床病例队列的测试。对乳酸酸中毒和蹄叶炎潜在的微生物动力学的新理解可能会导致更好的健康模型，改进的饲料配方和管理实践，以及开发用于患病马的益生菌治疗，其可用于预防和/或治疗每年影响数百万匹马的病症。这些细菌可以掺入动物饲料中，以防止引发蹄叶炎的发酵事件，这可能有助于通过减少牛蹄叶炎病例的数量来改善牛肉和乳制品生产，并降低马主的医疗费用。