CAREER: A low cost, label-free, multiplexable optical cavity biosensor with high sensitivity, high selectivity, and a large dynamic range using chained differential detection

疾病的早期发现是提高患者存活率和减少经济影响的关键。床旁诊断是通过在患者附近放置有效的诊断工具来实现疾病早期检测的时间关键解决方案，无论是在患者处还是在患者处？在床边还是在医生那里？的办公室，以监测和诊断病人？在使用集中实验室时，无需昂贵和耗时的过程，即可确保患者的健康和健康。本研究的目的是开发一种新型的光学腔生物传感器，使用链式差分检测的目的点的护理诊断。基于光学腔的生物传感器由于其无标记操作、低成本、高灵敏度和高选择性而本质上非常有希望用于护理点诊断。除了基于光学腔的生物传感器的固有优势之外，该团队还介绍了（1）三个激光二极管系统以实现多路复用，（2）差分检测方法以提高其灵敏度，（3）链式机制以显着增加线性动态范围，以及（4）简单的腔结构，易于与微流体等样品处理系统集成。成功完成拟议的研究项目将导致一个有前途的平台，可用于早期检测疾病的护理点生物传感器，并可能创造一个新的医学诊断模式。对于公众：本研究的目标是开发一种低成本和高灵敏度的设备，将测试多种疾病使用少量的生物样本，通常是血液。该设计由具有简单的两个反射镜结构（光学谐振腔）的生物传感器组成，该生物传感器使用众所周知的制造工艺构造。该设备的简单设计确保了其低成本，从而使发达国家和发展中国家的患者都能使用。由于早期发现疾病是有效治疗疾病的关键，因此定期对患者进行检测至关重要。该设备将使测试能够由患者在家中或在医生处进行？在诊所，代替广泛和耗时的测试，让病人定期监测多种疾病的存在。使患者能够定期检查自己将使疾病能够及早发现，并允许更有效和更便宜的治疗。

1350648 - KimTechnical AbstractThe early detection of diseases is the key to improving survival rates of patients and reducing economic impacts. Point of care diagnosis is the time critical solution for the early detection of diseases accomplished by staging effective diagnosis tools near the patient, either at the patient?s bedside or at a physician?s office, to monitor and diagnose the patient?s health and wellness without the costly and time-consuming processes necessary when using centralized laboratories. The objective of this proposed research is to develop a novel optical cavity biosensor using chained differential detection for the purpose of point of care diagnosis. Optical cavity based biosensors are by nature very promising for point of care diagnosis due to their label-free operation, low cost, high sensitivity, and high selectivity. On top of the intrinsic benefits of optical cavity based biosensors, the team introduces (1) a three laser diode system to accomplish multiplexability, (2) a differential detection method to enhance its sensitivity, (3) a chained mechanism to increase the linear dynamic range significantly, and (4) a simple cavity structure which is easy to integrate with a sample handling system such as microfluidics. Successful completion of the proposed research project will result in a promising platform for a point of care biosensor that can be used for early detection of diseases and possibly create a new paradigm of medical diagnosis.For general public:The goal of this research is to develop a low cost and highly sensitive device which will test for multiple diseases using a small amount of a biological sample, usually blood. The design is composed of a biosensor with simple two reflecting mirror structure (optical resonance cavity), constructed using well known fabrication processes. The simple design of the device ensures that it will be low cost, thus making it accessible to patients in both developed and developing countries. Since the early detection of diseases is the key to treating them effectively, it is crucial for patients to be tested regularly. This device will enable tests to be conducted by the patient in the home or at a physician?s office, in lieu of extensive and time consuming tests, allowing the patient to regularly monitor for the presence of multiple diseases. Enabling the patient to test his/herself regularly will allow diseases to be discovered early and allow for more effective and less expensive treatments.