CAREER: A low cost, label-free, multiplexable optical cavity biosensor with high sensitivity, high selectivity, and a large dynamic range using chained differential detection

摘要疾病的早期发现是提高患者存活率和减少经济影响的关键。护理点诊断是一种时间关键的解决方案，通过在患者附近，或者在患者身边，分期有效的诊断工具来完成疾病的早期发现。是在床边还是在医生面前？对病人进行监测和诊断？在使用集中式实验室时，无需进行昂贵且耗时的流程，就能促进健康和保健。本研究的目的是开发一种新型的光学腔生物传感器，使用链式差分检测用于护理点诊断。基于光学腔的生物传感器由于其无标签操作、低成本、高灵敏度和高选择性，在点护理诊断方面具有很大的前景。除了光学腔生物传感器固有的优点之外，该团队引入了(1)三个激光二极管系统来实现多路复用，(2)差分检测方法来提高其灵敏度，(3)链式机构来显着增加线性动态范围，以及(4)一个简单的腔结构，易于与样品处理系统（如微流体）集成。拟议的研究项目的成功完成将产生一个有希望的护理点生物传感器平台，可用于疾病的早期检测，并可能创造一种新的医学诊断范例。对于普通大众：这项研究的目标是开发一种低成本和高灵敏度的设备，它将使用少量的生物样本（通常是血液）来检测多种疾病。该设计由一个具有简单的双反射镜结构（光学谐振腔）的生物传感器组成，采用已知的制造工艺构建。该设备的简单设计确保了它的低成本，从而使发达国家和发展中国家的患者都可以使用它。由于疾病的早期发现是有效治疗的关键，因此对患者进行定期检查至关重要。这种设备可以让病人在家里或医生那里进行测试。代替了大量耗时的检查，使患者能够定期监测多种疾病的存在。使病人能够定期对自己进行检查，将有助于及早发现疾病，并使治疗更有效、更便宜。

1350648 - KimTechnical AbstractThe early detection of diseases is the key to improving survival rates of patients and reducing economic impacts. Point of care diagnosis is the time critical solution for the early detection of diseases accomplished by staging effective diagnosis tools near the patient, either at the patient?s bedside or at a physician?s office, to monitor and diagnose the patient?s health and wellness without the costly and time-consuming processes necessary when using centralized laboratories. The objective of this proposed research is to develop a novel optical cavity biosensor using chained differential detection for the purpose of point of care diagnosis. Optical cavity based biosensors are by nature very promising for point of care diagnosis due to their label-free operation, low cost, high sensitivity, and high selectivity. On top of the intrinsic benefits of optical cavity based biosensors, the team introduces (1) a three laser diode system to accomplish multiplexability, (2) a differential detection method to enhance its sensitivity, (3) a chained mechanism to increase the linear dynamic range significantly, and (4) a simple cavity structure which is easy to integrate with a sample handling system such as microfluidics. Successful completion of the proposed research project will result in a promising platform for a point of care biosensor that can be used for early detection of diseases and possibly create a new paradigm of medical diagnosis.For general public:The goal of this research is to develop a low cost and highly sensitive device which will test for multiple diseases using a small amount of a biological sample, usually blood. The design is composed of a biosensor with simple two reflecting mirror structure (optical resonance cavity), constructed using well known fabrication processes. The simple design of the device ensures that it will be low cost, thus making it accessible to patients in both developed and developing countries. Since the early detection of diseases is the key to treating them effectively, it is crucial for patients to be tested regularly. This device will enable tests to be conducted by the patient in the home or at a physician?s office, in lieu of extensive and time consuming tests, allowing the patient to regularly monitor for the presence of multiple diseases. Enabling the patient to test his/herself regularly will allow diseases to be discovered early and allow for more effective and less expensive treatments.