CAREER: Development of Mesoporous Metal-Organic Frameworks as a New Type of Platform for Enzyme Immobilization

职业：开发介孔金属有机框架作为新型酶固定化平台

• 批准号: 1352065
• 负责人: Shengqian Ma
• 金额: $ 57.5万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-03-15 至 2020-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1352065&HistoricalAwards=false
• 关键词: CAREER; Development; Mesoporous; Metal; Organic

TECHNICAL SUMMARY:With the support of the Solid State and Materials Chemistry program in the Division of Materials Research, Professor Shengqian Ma at the University of South Florida will develop mesoporous metal-organic frameworks (mesoMOFs) as a new type of host matrix materials for enzyme immobilization, which will not only circumvent the drawbacks of existing host matrix materials but also offer new platforms for "tunable" enzyme encapsulation. The aims of the proposed research include (i) design and synthesis of water-stable functional mesoMOFs; (ii) development of protocols to immobilize enzymes into mesoMOFs, characterizations of enzymes@mesoMOFs, and evaluation of their performances in enzymatic catalysis; and (iii) tailoring functional mesoMOFs as platforms for chemo-enzymatic and multi-enzymatic cascade catalysis. Mechanistic studies will also be performed to investigate the behavior of enzyme encapsulated within the mesoMOFs and to unveil the specific interactions between enzyme molecules and mesoMOF frameworks. NON-TECHNICAL SUMMARY:With the support of the Solid State and Materials Chemistry program in the Division of Materials Research, the proposed research aims to advance mesoporous metal-organic frameworks as a new type of host matrix materials for enzyme immobilization, which would impact chemical/fine chemical, pharmaceutical, and food industries: it will formulate new methodologies to construct functional metal-organic framework materials for a wider range of applications and help our understanding of enzymatic reactions in nature. The award will help the PI to establish a multidisciplinary research program of materials, solid-state chemistry, catalysis and biochemistry that will lead to the discovery of novel functional materials with profound relevance to energy sustainability and green chemistry. The proposed research will thereby provide the type of professional scientific training that would be desirable for STEM students. The integrated research and educational activities and proposed outreach programs will specifically target participation of minorities and underrepresented groups thus providing a platform to motivate and foster them for pursuing careers in STEM fields.

技术总结：在材料研究部固态与材料化学项目的支持下，南佛罗里达大学的马胜谦教授将开发介孔金属有机框架（mesoMOFs）作为一种新型的酶固定化主体基质材料，这不仅可以规避现有主体基质材料的缺点，还可以为“可调”的酶封装提供新的平台。拟议研究的目标包括：（i）设计和合成水稳定的功能mesoMOFs;（ii）开发将酶转化为mesoMOFs的方案，表征酶@mesoMOFs，并评估其在酶催化中的性能;以及（iii）定制功能mesoMOFs作为化学-酶和多酶级联催化的平台。还将进行机理研究以研究包封在mesoMOF内的酶的行为，并揭示酶分子与mesoMOF框架之间的特异性相互作用。非技术性总结：在材料研究部固态和材料化学项目的支持下，拟议的研究旨在推进介孔金属有机框架作为一种新型的酶固定化基质材料，这将影响化学/精细化工，制药和食品工业：它将制定新的方法来构建功能金属有机框架材料，用于更广泛的应用，并帮助我们理解自然界中的酶反应。该奖项将帮助PI建立一个材料，固态化学，催化和生物化学的多学科研究计划，这将导致发现与能源可持续性和绿色化学具有深远意义的新型功能材料。因此，拟议的研究将提供STEM学生所需的专业科学培训类型。综合研究和教育活动以及拟议的外展计划将专门针对少数民族和代表性不足的群体的参与，从而提供一个平台，激励和促进他们在STEM领域追求职业生涯。