Molecular mechanisms underlying temperature modulation of plant immunity

植物免疫温度调节的分子机制

• 批准号: 1353738
• 负责人: Jian Hua
• 金额: $ 62.86万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353738&HistoricalAwards=false
• 关键词: Molecular; mechanisms; underlying; temperature; modulation

Broader significancePlant immune responses are greatly modulated by environmental signals including temperature, and this modulation is an adaptive mechanism for a balanced environmental response and plant growth. There is limited knowledge on how temperature is perceived and how it regulates plant immune responses. This project will identify targets of temperature on immune responses and reveal major strategies plants use to tune immunity to temperature. This project will enhance the prediction power of climate change on plant growth and defense and will provide guidance in breeding crop plants that have a balanced growth and defense for the changing environment. The project will integrate education with research for undergraduate students including minorities at and outside Cornell through various programs including NSF Plant Genome REU Site. One of the four aims of the project will be conducted mostly by undergraduates. This project will also provide a broad training to a postdoctoral fellow and a graduate student in research and teaching assisted by the NSF ?Center for the integration of research, teaching, and learning? program. Discoveries generated from this project will be disseminated through journal publications, conference presentations and classroom teaching, thus contributing to the advancement of science.Technical DescriptionThe goal of this project is to further elucidate the molecular mechanisms underlying temperature modulation of immune receptor R-mediated immune responses. Earlier studies revealed that R proteins could function as temperature sensors and deficiency of abscisic acid antagonizes the high temperature effects on R proteins. This project will first further reveal how temperature and abscisic acid regulate the activity of R proteins to affect immunity. This will be achieved by dissecting the regulation through genetic screens using Arabidopsis autoimmune plants. Mutants with altered temperature sensitivity in immune responses will be isolated and cloned, and the mode of gene action will be investigated. This project will also reveal the genetic basis of variation of temperature sensitivity of immunity in natural populations. This will be achieved by surveying wild Arabidopsis accessions for their temperature sensitivities in disease resistance. Major loci determining the temperature sensitivity will be isolated. With identification of mode of regulation of R proteins by temperature and ABA as well as major strategies in tuning immunity to temperature signals, this project will reveal new mechanisms of signal sensing and signal integration and provide strategies to generate plants with optimal responses to a changing environment.

植物的免疫反应受到包括温度在内的环境信号的极大调节，这种调节是平衡环境反应和植物生长的适应机制。关于温度如何被感知以及它如何调节植物免疫反应的知识有限。该项目将确定温度对免疫反应的影响，并揭示植物用于调节免疫力以适应温度的主要策略。该项目将提高气候变化对植物生长和防御的预测能力，并将为培育具有平衡生长和防御变化环境的作物植物提供指导。该项目将通过包括NSF植物基因组REU网站在内的各种计划，将教育与研究结合起来，为包括康奈尔大学内外少数民族在内的本科生提供教育。该项目的四个目标之一将主要由本科生进行。该项目还将提供一个广泛的培训，以博士后研究员和研究生的研究和教学的国家科学基金会的援助？研究、教学和学习一体化中心？程序.本项目的研究成果将通过期刊出版、会议报告和课堂教学等方式传播，从而促进科学的进步。技术说明本项目的目标是进一步阐明免疫受体R介导的免疫反应的温度调节的分子机制。早期的研究表明，R蛋白具有温度传感器的功能，脱落酸缺乏可拮抗高温对R蛋白的影响。该项目将首先进一步揭示温度和脱落酸如何调节R蛋白的活性以影响免疫力。这将通过使用拟南芥自身免疫植物通过遗传筛选剖析调节来实现。将分离和克隆在免疫反应中具有改变的温度敏感性的突变体，并研究基因作用的模式。本项目还将揭示自然群体免疫温度敏感性变异的遗传基础。这将通过调查野生拟南芥种质在抗病性方面的温度敏感性来实现。将分离决定温度敏感性的主要基因座。通过鉴定温度和阿坝对R蛋白的调节模式以及调节免疫对温度信号的主要策略，该项目将揭示信号感知和信号整合的新机制，并提供策略以产生对变化的环境具有最佳响应的植物。

项目成果

AIG2A and AIG2B limit the activation of salicylic acid-regulated defenses by tryptophan-derived secondary metabolism in Arabidopsis

AIG2A和AIG2B通过拟南芥中色氨酸衍生的次级代谢限制水杨酸调节的防御的激活

• DOI: 10.1093/plcell/koac255
• 发表时间: 2022
• 期刊: The Plant Cell
• 作者: Wang, Zhixue;Yang, Leiyun;Jander, Georg;Bhawal, Ruchika;Zhang, Sheng;Liu, Zhenhua;Oakley, Aaron;Hua, Jian
• 通讯作者: Hua, Jian

Multiple chromatin-associated modules regulate expression of an intracellular immune receptor gene in Arabidopsis

多个染色质相关模块调节拟南芥细胞内免疫受体基因的表达

• 发表时间: 2022
• 期刊: New phytologist
• 影响因子: 9.4
• 作者: Leiyun Yang, Zhixue Wang