COLLABORATIVE RESEARCH: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing Radiation

提案：1403860/1403053 PI：Rege/Hayes机构：亚利桑那州州立大学/蒙大拿州标题：多肽模板纳米粒子作为电离辐射的比色传感器电离辐射，包括X射线和伽马射线，在几个医疗，国防，空间和农业应用中是有用的。然而，不受控制的电离辐射可能导致大规模的灾害和对人类健康的损害。考虑到平衡潜在有害影响和广泛应用的必要性，重要的是开发新的辐射传感方法，以确保安全和防止事故。我们最近开发了一种新的纳米生物传感方法，其中我们采用蛋白质作为模板，从无色金属盐中形成金属纳米颗粒。这种从无色金属盐到纳米粒子在水中的有色分散体的变化是电离辐射的一个简单而直观的指标。在拟议的研究中，我们将对不同的氨基酸和小肽如何在不同剂量的电离辐射存在下介导纳米颗粒的形成进行基础研究。实验和数学建模的方法将被用来研究这种行为。我们的初步结果表明，多肽能够在电离辐射（X射线）的存在下从无色金属盐模板化等离子体金属纳米颗粒。在拟议的研究中，我们将对20种天然氨基酸模板中的每一种如何在暴露于不同剂量的电离辐射时使金银纳米粒子结合进行广泛的调查。然后，我们将研究二肽（？最小肽？），其中具有模板化等离子体纳米颗粒形成活性的氨基酸与可以结合和稳定模板化纳米颗粒的氨基酸并置。此外，还将研究其中结合和模板化氨基酸由三甘氨酸接头分离的五肽。将采用数学建模以研究不同的氨基酸和肽以及实验物理化学条件如何影响纳米颗粒的形成动力学。水辐解，金属离子还原，和纳米粒子的形成将建模，pH值，清除剂，和模板生物分子的影响将进行研究。实验和建模研究将用于分子工程和开发基于多肽的电离辐射比色纳米生物传感器的序列-性质关系。

Proposal: 1403860/1403053PI: Rege/HayesInstitution: Arizona State University/ Montana StateTitle: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing RadiationIonizing radiation, including X-rays and gamma rays, is useful in several medical, defense, space, and agricultural applications. However, uncontrolled ionizing radiation can lead to large scale disasters and damage to human health. Given the necessity to balance potential deleterious effects and widespread application, it is important to develop novel radiation-sensing methods in order to ensure safety and prevent accidents. We have recently developed a novel nanobiosensing approach in which we employed proteins for templating the formation of metal nanoparticles from colorless metal salts. This change from colorless metal salts to colored dispersions of nanoparticles in water is a simple and visual indicator of ionizing radiation. In the proposed research, we will carry out a fundamental study on how different amino acids and small peptides mediate nanoparticle formation in the presence of different doses of ionizing radiation. Both, experimental and mathematical modeling approaches will be employed to study this behavior. Our preliminary results indicated that polypeptides are capable of templating plasmonic metal nanoparticles from colorless metal salts in presence of ionizing radiation (X-Rays). In the proposed research, we will carry out an extensive investigation into how each of the 20 natural amino acids template bimetallic gold-silver nanoparticles upon exposure to different doses of ionizing radiation. We will then investigate dipeptides (?minimal peptides?), in which an amino acid with activity for templating the formation of plasmonic nanoparticles is juxtaposed with an amino acid that can bind and stabilize the templated nanoparticles. In addition, pentapeptides in which the binding and templating amino acids are separated by a tri-glycine linker will also be investigated. Mathematical modeling will be employed in order to investigate how different amino acids and peptides, together with experimental physicochemical conditions influence the formation kinetics of bimetallic nanoparticles. Water radiolysis, metal ion reduction, and nanoparticle formation will be modeled, and effects of pH, scavenging agents, and templating biomolecules will be investigated. Experimental and modeling studies will be employed for molecular engineering and developing sequence-property relationships for polypeptide-based colorimetric nanobiosensors for ionizing radiation.