COLLABORATIVE RESEARCH: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing Radiation

合作研究：多肽模板纳米粒子作为电离辐射比色传感器

• 批准号: 1403860
• 负责人: Kaushal Rege
• 金额: $ 29.96万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1403860&HistoricalAwards=false
• 关键词: COLLABORATIVE; RESEARCH; Polypeptide; Templated; Nanoparticles

Proposal: 1403860/1403053PI: Rege/HayesInstitution: Arizona State University/ Montana StateTitle: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing RadiationIonizing radiation, including X-rays and gamma rays, is useful in several medical, defense, space, and agricultural applications. However, uncontrolled ionizing radiation can lead to large scale disasters and damage to human health. Given the necessity to balance potential deleterious effects and widespread application, it is important to develop novel radiation-sensing methods in order to ensure safety and prevent accidents. We have recently developed a novel nanobiosensing approach in which we employed proteins for templating the formation of metal nanoparticles from colorless metal salts. This change from colorless metal salts to colored dispersions of nanoparticles in water is a simple and visual indicator of ionizing radiation. In the proposed research, we will carry out a fundamental study on how different amino acids and small peptides mediate nanoparticle formation in the presence of different doses of ionizing radiation. Both, experimental and mathematical modeling approaches will be employed to study this behavior. Our preliminary results indicated that polypeptides are capable of templating plasmonic metal nanoparticles from colorless metal salts in presence of ionizing radiation (X-Rays). In the proposed research, we will carry out an extensive investigation into how each of the 20 natural amino acids template bimetallic gold-silver nanoparticles upon exposure to different doses of ionizing radiation. We will then investigate dipeptides (?minimal peptides?), in which an amino acid with activity for templating the formation of plasmonic nanoparticles is juxtaposed with an amino acid that can bind and stabilize the templated nanoparticles. In addition, pentapeptides in which the binding and templating amino acids are separated by a tri-glycine linker will also be investigated. Mathematical modeling will be employed in order to investigate how different amino acids and peptides, together with experimental physicochemical conditions influence the formation kinetics of bimetallic nanoparticles. Water radiolysis, metal ion reduction, and nanoparticle formation will be modeled, and effects of pH, scavenging agents, and templating biomolecules will be investigated. Experimental and modeling studies will be employed for molecular engineering and developing sequence-property relationships for polypeptide-based colorimetric nanobiosensors for ionizing radiation.

提案：1403860/1403053PI：REGE/Hayes机构：亚利桑那州立大学/蒙大拿州标题：多肽模板纳米颗粒作为电离辐射的比色传感器包括X射线和伽马射线，在医疗、国防、太空和农业应用中非常有用。然而，不受控制的电离辐射会导致大规模灾难和对人类健康的损害。考虑到平衡潜在有害影响和广泛应用的必要性，开发新的辐射传感方法以确保安全和防止事故是重要的。我们最近开发了一种新的纳米生物传感方法，其中我们使用蛋白质来从无色金属盐中形成金属纳米颗粒的模板。这种从无色金属盐到纳米颗粒在水中有色分散的变化是电离辐射的一个简单而直观的指示器。在这项拟议的研究中，我们将开展一项基础研究，即不同的氨基酸和小肽如何在不同剂量的电离辐射存在下介导纳米粒子的形成。实验和数学建模方法都将被用来研究这种行为。我们的初步结果表明，在电离辐射(X-射线)存在的情况下，多肽能够从无色金属盐中模板形成血浆金属纳米颗粒。在这项拟议的研究中，我们将对20种天然氨基酸在不同剂量的电离辐射下如何模板双金属金-银纳米颗粒进行广泛的调查。然后我们将研究二肽(最小多肽)，其中具有模板形成血浆纳米颗粒的活性的氨基酸与能够结合和稳定模板纳米颗粒的氨基酸并列。此外，还将研究通过三甘氨酸连接物分离结合氨基酸和模板氨基酸的五肽。数学模型将被用来研究不同的氨基酸和多肽，以及实验的物理化学条件如何影响双金属纳米颗粒的形成动力学。将模拟水的辐解、金属离子还原和纳米颗粒的形成，并将研究pH、清除剂和模板生物分子的影响。实验和模型研究将用于分子工程和开发基于多肽的电离辐射比色纳米生物传感器的序列-性质关系。