I-Corps: Two-Bead Method for Enhanced Cell Isolation from Complex Samples

I-Corps：两珠法增强复杂样品中的细胞分离

• 批准号: 1405538
• 负责人: John Younger
• 金额: $ 5万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-03-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1405538&HistoricalAwards=false
• 关键词: Corps; Two; Bead; Method; Enhanced

The team from the University of Michigan has developed a new technology that allows purification of cells, based on the simultaneous antibody detection of any two distinct surface features, called two-particle activated cell sorting. This is important because biological cell research and medical technologies require efficient cell separation techniques to advance basic research of cells and for medical research and clinical treatment. Current approaches include fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS). FACS requires expensive instrumentation and MACS lacks specificity.Being able to enrich low levels of cells to a high degree of purity from complex samples is recognized as being a significant missing component in many areas of life science. The proposed work plans on meeting this need through a combination of two-surface marker isolation (currently only possible with expensive instrumentation) with the ease of use and cost of magnetic separation (currently only possible to perform single-parameter separation). This technology may have a potential to shift the market landscape creating a niche opportunity between FACS and MACS, only taking the advantage of both.

来自密歇根大学的研究小组开发了一种新技术，该技术可以基于任何两种不同表面特征的同时抗体检测来纯化细胞，称为双粒子激活细胞分选。这是重要的，因为生物细胞研究和医疗技术需要有效的细胞分离技术来推进细胞的基础研究以及用于医学研究和临床治疗。目前的方法包括荧光激活细胞分选（FACS）和磁激活细胞分选（MACS）。FACS需要昂贵的仪器，而MACS缺乏特异性。能够从复杂样品中富集低水平的细胞到高纯度，被认为是生命科学许多领域中的重要组成部分。拟议的工作计划是通过将两个表面的标记物分离（目前只有昂贵的仪器才能做到）与磁分离（目前只能进行单参数分离）的易用性和成本相结合来满足这一需要。这项技术可能有潜力改变市场格局，在FACS和MACS之间创造一个利基机会，只有利用两者的优势。