I-Corps: Two-Bead Method for Enhanced Cell Isolation from Complex Samples

密歇根大学的研究小组开发了一种新技术，可以基于对任何两个不同表面特征的同时抗体检测来净化细胞，称为两粒子激活细胞分选。这一点很重要，因为生物细胞研究和医疗技术需要有效的细胞分离技术来推进细胞的基础研究，并用于医学研究和临床治疗。目前的方法包括荧光激活细胞分选(FACS)和磁激活细胞分选(MACS)。流式细胞仪需要昂贵的仪器，而MACs缺乏特异性。能够从复杂的样本中浓缩低水平的细胞到高纯度被认为是生命科学许多领域中一个重要的缺失成分。拟议的工作计划是通过结合双表面标记隔离(目前只有昂贵的仪器才能实现)和磁分离的易用性和成本(目前只能进行单参数分离)来满足这一需要。这项技术可能会改变市场格局，在FACS和Mac之间创造一个利基机会，只是利用了两者的优势。

The team from the University of Michigan has developed a new technology that allows purification of cells, based on the simultaneous antibody detection of any two distinct surface features, called two-particle activated cell sorting. This is important because biological cell research and medical technologies require efficient cell separation techniques to advance basic research of cells and for medical research and clinical treatment. Current approaches include fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS). FACS requires expensive instrumentation and MACS lacks specificity.Being able to enrich low levels of cells to a high degree of purity from complex samples is recognized as being a significant missing component in many areas of life science. The proposed work plans on meeting this need through a combination of two-surface marker isolation (currently only possible with expensive instrumentation) with the ease of use and cost of magnetic separation (currently only possible to perform single-parameter separation). This technology may have a potential to shift the market landscape creating a niche opportunity between FACS and MACS, only taking the advantage of both.