DISSERTATION RESEARCH: Effects of concurrent thermal, osmotic and toxicant stress on the copepod Tigriopus californicus

论文研究：同时发生的热、渗透和毒物应激对桡足类虎纹虎的影响

• 批准号: 1406306
• 负责人: Suzanne Edmands
• 金额: $ 2.13万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1406306&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Effects; concurrent; thermal

We will explore how organisms respond and adapt to complex, stressful environments by examining the gene activity of a marine invertebrate, the copepod Tigriopus californicus. When challenged with copper, temperature and salinity stress, we observe different levels of survivorship: more individuals from a southern population are likely to survive than from a northern population. We will compare the set of active genes (RNA) specific to the responses of two populations under both single and combined stress. This research explores the consequences of general and specific stress responses: we hypothesize that some genes respond to just one stress while other genes are part of a shared response system. Interaction between these responses could reduce the effect of an additional stress or compound the problem. In resilient and vulnerable populations, we will observe differences in gene activity that may explain how they try to adapt to multiple challenges. Linking the results of this project with genetic analyses will frame adaptation and stress-tolerance, comparing both the gene and its activity response. This project will answer critical questions about how species deal with stressful environments that present simultaneous challenges. Exploring population-wide variation and the context of stressful natural environments will be key to understanding organism-habitat interactions, and is increasingly important in light of climate changes. Integrative approaches that identify these compounded responses and the biological mechanisms at play will provide a robust context for understanding the effects of pollution and contamination. This research will also identify genetic activity that makes one population more resilient than another in complex environments.

我们将通过检测海洋无脊椎动物--剑齿虎的基因活性，来探索生物如何对复杂的压力环境做出反应和适应。当受到铜、温度和盐度压力的挑战时，我们观察到不同水平的生存：来自南方种群的个体可能比来自北方种群的个体更有可能存活下来。我们将比较两个种群在单一和复合压力下的反应所特有的活性基因(RNA)集。这项研究探索了一般和特定压力反应的后果：我们假设一些基因只对一种压力做出反应，而另一些基因是共同反应系统的一部分。这些反应之间的相互作用可能会减少额外压力的影响，或者使问题复杂化。在有弹性和脆弱的人群中，我们将观察到基因活性的差异，这可能解释了他们如何努力适应多重挑战。将该项目的结果与遗传分析联系起来，将构建适应和压力耐受性框架，比较基因及其活动反应。这个项目将回答有关物种如何应对同时存在挑战的压力环境的关键问题。探索种群范围的变异和紧张的自然环境的背景将是理解生物-栖息地相互作用的关键，鉴于气候变化，这一点越来越重要。确定这些复合反应和发挥作用的生物机制的综合方法将为理解污染和污染的影响提供强有力的背景。这项研究还将确定使一个种群在复杂环境中比另一个种群更具弹性的遗传活动。