A phylogenetic approach to understanding the evolution of hybrid viability loci in Tigriopus californicus

一种了解加州虎杂交活力基因座进化的系统发育方法

• 批准号: 1146520
• 负责人: Suzanne Edmands
• 金额: $ 17.3万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146520&HistoricalAwards=false
• 关键词: phylogenetic; approach; understanding; evolution; hybrid

The copepod Tigriopus californicus is an emerging model system for studies of speciation and resistance to environmental stress. Such work will be greatly improved by a better understanding of the evolutionary relationships among T. californicus populations. The current study will construct an evolutionary tree (phylogeny) of 20 populations using DNA sequence from 12 genes. A number of different methods will be used to determine the best evolutionary tree for the combined dataset, including analyses of the level of agreement among different genes. Results will be used to test the null hypothesis that genes evolved at equal rates, with the expectation that nuclear genes (chromosomal genes inherited from both parents) evolved more slowly than mitochondrial genes (genes on a separate organelle inherited only from the mother). The completed phylogeny will provide an evolutionary framework for future planned studies of how gene conflicts accumulate when isolated populations diverge from each other.DNA sequence data will be deposited in public databases, thereby increasing genomic resources for copepods, one of the most abundant groups of multicellular animals on the planet. Beyond its relevance to copepods, the research will contribute to the broader issue of the rate of evolutionary change in mitochondrial vs. nuclear genes. The work will directly fund research by both graduate and undergraduate students. Funds will also be used to support a new collaboration between the Edmands Lab and the Aquarium of the Pacific aimed at enhancing public understanding of issues involving evolution in marine environments.

加州虎足虫是研究物种形成和环境胁迫抗性的一个新兴模式系统。对T.加州人口。目前的研究将使用12个基因的DNA序列构建一个20个种群的进化树（进化树）。将使用许多不同的方法来确定组合数据集的最佳进化树，包括分析不同基因之间的一致性水平。结果将用于检验基因以相同速率进化的零假设，期望核基因（从父母双方遗传的染色体基因）比线粒体基因（仅从母亲遗传的单独细胞器上的基因）进化得更慢。完成的基因组学将为未来计划的研究提供一个进化框架，研究当孤立的种群彼此分化时基因冲突如何积累。DNA序列数据将存放在公共数据库中，从而增加桡足类的基因组资源，桡足类是地球上最丰富的多细胞动物群体之一。除了与桡足类动物的相关性外，这项研究还将有助于解决线粒体与核基因进化变化率的更广泛问题。这项工作将直接资助研究生和本科生的研究。资金还将用于支持Edmands实验室和太平洋水族馆之间的新合作，旨在提高公众对海洋环境演变问题的认识。