EAPSI: Antimicrobial effects of gold and silver nanoparticles

EAPSI：金银纳米颗粒的抗菌作用

• 批准号: 1414982
• 负责人: Elaheh Kamaloo
• 金额: $ 0.51万
• 依托单位: Kamaloo Elaheh
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1414982&HistoricalAwards=false
• 关键词: EAPSI; Antimicrobial; effects; gold; silver

Bacterial contamination affects industrial processes, food processing equipment, biomaterials, and other biomedical contamination. The ability to control bacterial adhesion on surfaces and to kill harmful bacteria requires that new methodologies are developed. Over the last decades, the resistance of pathogenic bacteria to antimicrobial agents has become a major challenge. A novel approach would be to use a new class of nano-antibiotics, which can kill bacteria without promoting antimicrobial resistance. The goal of this project is to study how silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) can be exploited as antibacterial agents. Among different metals, silver is known for its antibacterial and growth inhibitory effects. Gold does not show any bactericidal effect in bulk amount. However at the nanoscale, gold NPs in a certain size range have some unique properties that make them good candidates for killing bacteria through pore formation in bacterial membranes. A great benefit of this research is that since AgNPs and AuNPs kill bacteria through membrane-associated processes, there is a low likelihood of bacteria being able to develop a resistance to their actions. This project will be conducted under supervision of Dr. Jonghoon Choi, a noted expert on nano-biotechnology, at Hanyang University in Korea.In this project the antimicrobial effect of gold and silver nanoparticles (NPs) on Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli) will be characterized. NP solutions of varying sizes and concentrations will be prepared. Morphology and size of NPs will be characterized by transmission electron microscopy (TEM) and light scattering. Electrostatic charge of NPs will be characterized via zeta potential measurements. Minimum Inhibitory Concentration (MIC) of NPs on E. coli and S. aureus will be determined by using plate counting and growth rate experiments. This NSF EAPSI award is funded in collaboration with the National Research Foundation of Korea.

细菌污染影响工业流程、食品加工设备、生物材料和其他生物医学污染。控制表面细菌粘附和杀死有害细菌的能力需要开发新的方法。在过去的几十年里，病原菌对抗菌药物的耐药性已成为一个重大挑战。一种新颖的方法是使用新型纳米抗生素，它可以杀死细菌而不增加抗菌素耐药性。该项目的目标是研究如何利用银纳米颗粒（AgNP）和金纳米颗粒（AuNP）作为抗菌剂。在不同的金属中，银以其抗菌和生长抑制作用而闻名。大量黄金没有表现出任何杀菌作用。然而，在纳米尺度上，一定尺寸范围内的金纳米粒子具有一些独特的性质，使它们成为通过在细菌膜上形成孔来杀死细菌的良好候选者。这项研究的一大好处是，由于 AgNP 和 AuNP 通过膜相关过程杀死细菌，因此细菌对其作用产生抵抗力的可能性很低。该项目将在韩国汉阳大学著名纳米生物技术专家 Jonghoon Choi 博士的监督下进行。在该项目中，将表征金银纳米颗粒 (NP) 对金黄色葡萄球菌 (S. aureus) 和大肠杆菌 (E. coli) 的抗菌作用。将制备不同尺寸和浓度的纳米颗粒溶液。纳米颗粒的形态和尺寸将通过透射电子显微镜（TEM）和光散射进行表征。纳米粒子的静电电荷将通过 zeta 电位测量来表征。纳米颗粒对大肠杆菌和金黄色葡萄球菌的最低抑制浓度 (MIC) 将通过平板计数和生长速率实验来确定。该 NSF EAPSI 奖项是与韩国国家研究基金会合作资助的。