Molecular and physiological characterization of members of the aluminium-activated malate transporter (ALMT) family with respect to their contribution to mineral nutrition in rice.

铝激活苹果酸转运蛋白 (ALMT) 家族成员的分子和生理特征及其对水稻矿物质营养的贡献。

• 批准号: 215611924
• 负责人: Dr. Hendrik Führs
• 金额: --
• 依托单位: CSIRO Research Division of Plant Industry
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/215611924?language=en
• 关键词: Molecular; physiological; characterization; members; aluminium

Aluminium-activated malate transporter (ALMT) proteins have been shown to act as malate translocators across membranes. The role of specific ALMT family members in Aluminium (Al) tolerance and stomata function has been clarified, but the role of the remaining members of the ALMT family remains unknown. The aim of the project is to identify and characterize those members of the ALMT family having an impact on the mineral nutrition of rice. Such an impact might occur either through direct processes such as transport of mineral anions or by indirect processes that involve the transport of organic anions such as malate. To clarify the nature of the contribution of ALMT family members to rice mineral nutrition the project is divided into three main parts: (1) characterization of the mineral nutrition phenotype of existing transgenic rice lines generated in the host laboratory, (2) characterization of appropriate rice mutant lines from stock centers with respect to a mineral nutrition phenotype, and (3) ALMT localization studies through generation of promoter:GFP transgenic rice lines and transient protein:GFP fusions. In sum, this project concept represents a mix of characterizing several OsALMTs with respect to their contribution to mineral nutrition on the basis of existing germplasm and laying a basis for future work on the characterization of further OsALMT family members.

已证明，谷氨酰胺激活的苹果酸转运蛋白（ALMT）蛋白可作为跨膜的苹果酸转运蛋白。特定ALMT家族成员在铝（Al）耐受性和气孔功能中的作用已被阐明，但ALMT家族的其余成员的作用仍然未知。该项目的目的是确定和表征ALMT家族中对水稻矿物质营养有影响的成员。这种影响可能通过矿物阴离子迁移等直接过程或涉及苹果酸盐等有机阴离子迁移的间接过程产生。为了阐明ALMT家族成员对水稻矿物质营养的贡献的性质，该项目分为三个主要部分：（1）鉴定宿主实验室中产生的现有转基因水稻品系的矿物质营养表型，（2）鉴定来自储备中心的适当水稻突变品系的矿物质营养表型，（3）通过构建启动子：GFP转基因水稻株系和瞬时蛋白：GFP融合体进行ALMT定位研究。总之，这个项目的概念代表了一个混合的表征几个OsALMT关于其贡献的矿物营养的基础上，现有的种质资源，并奠定了基础，为未来的工作进一步OsALMT家族成员的表征。