Exploring the physiological and ecological basis of mixotrophy in marine food webs
探索海洋食物网混合营养的生理和生态基础
基本信息
- 批准号:1436169
- 负责人:
- 金额:$ 61.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-10-01 至 2018-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Marine phytoplankton are responsible for about half of global primary production despite being seasonally or chronically nutrient limited. To cope with this, many phytoplankton supplement their nutritional needs through mixotrophy, which involves feeding on bacteria or other algae. These microscopic Venus Fly Traps of the ocean are major players in marine microbial food webs, yet we know so little about when they feed and how their eating is balanced with photosynthesis. This research will shed light on how environmental and cellular factors control mixotrophy, and how mixotrophy and photosynthesis are integrated in the overall metabolism. While understanding the ecological role of mixotrophy in ocean food webs is center to this work, results from this study will also shed light on the evolution of mixotrophy by identifying potential tradeoffs between feeding and photosynthesis. This work will involve collaboration with The Zephyr Education Foundation, a marine science literacy and education program, to design an educational unit to emphasize the importance of mixotrophy in marine microbial food webs, and will utilize boat trips and classroom activities in order to communicate these concepts. Undergraduate students from underrepresented groups will be trained at the Woods Hole Oceanographic Institution (minority fellowship program), as well as local high school summer students, to perform lab research on mixotrophs. This research will also provide training and career development for a postdoctoral scientist. Mixotrophy refers to species that combine some level of phagotrophy and phototrophy, and represents a diverse array of ecological interactions and cellular and metabolic adaptations. While often perceived as an exception to the norm, mixotrophy is commonplace in marine food webs, affording phytoplankton greater ecological fitness during periods of low or limiting nutrients while stabilizing food webs. Many mixotrophs have a low chlorophyll: carbon ratio, which tends to make them poor phototrophic competitors. In turn, feeding allows these species to achieve maximum growth while in some cases also eliminating their competitors. Other mixotrophs are strong phototrophic competitors, and only feed when severely nutrient limited. This research will determine the cellular and environmental factors that lead to feeding by marine phytoplankton, and how the contrasting metabolisms of heterotrophy and photosynthesis are integrated within a cell. This research will involve laboratory-based experiments on model dinoflagellate and chrysophyte cultures. Using microscopy, physiology, proteomics and metabolomics approaches, this work will test hypotheses about the ultimate causes and consequences of mixotrophy. The major objectives are to determine 1) environmental controls for inducing mixotrophy, 2) the role of prey quality on predator selection, 3) cellular and molecular controls of mixotrophy, and 4) nutrient assimilation and integrated metabolism. Using these various research approaches, this work will produce a comprehensive view of several mixotrophs and provide new insights into cellular, ecological, and evolutionary aspects of mixotrophy. Results from this research will improve our understanding of the physiological and ecological role of mixotrophy in marine phytoplankton, and provide much needed molecular markers for studying this process in both the laboratory and field.
海洋浮游植物尽管受到季节性或长期的营养限制,但仍占全球初级生产的一半左右。为了科普这一问题,许多浮游植物通过混合营养补充其营养需求,其中包括以细菌或其他藻类为食。海洋中这些微小的金星蝇陷阱是海洋微生物食物网的主要参与者,但我们对它们何时进食以及它们的进食如何与光合作用平衡知之甚少。这项研究将揭示环境和细胞因素如何控制混合营养,以及混合营养和光合作用如何整合到整体代谢中。虽然了解混合营养在海洋食物网中的生态作用是这项工作的中心,但这项研究的结果也将通过确定摄食和光合作用之间的潜在权衡来阐明混合营养的进化。这项工作将涉及与Zephyr教育基金会,海洋科学素养和教育计划合作,设计一个教育单元,强调海洋微生物食物网中混合营养的重要性,并将利用乘船旅行和课堂活动来传达这些概念。来自代表性不足群体的本科生将在伍兹霍尔海洋研究所(少数民族奖学金计划)接受培训,以及当地高中暑期学生,进行混合营养体的实验室研究。这项研究还将为博士后科学家提供培训和职业发展。混养是指联合收割机结合了一定程度的吞噬和光养的物种,代表了多种多样的生态相互作用以及细胞和代谢适应。虽然经常被视为一个例外的规范,混合营养是常见的海洋食物网,提供浮游植物在低或有限的营养期间,同时稳定食物网更大的生态适应性。许多混合营养生物的叶绿素与碳的比例很低,这往往使它们成为弱的光养竞争者。反过来,喂养使这些物种实现最大的增长,同时在某些情况下也消除了它们的竞争对手。其他混合营养体是强大的光养竞争者,仅在营养严重受限时进食。这项研究将确定导致海洋浮游植物摄食的细胞和环境因素,以及异养和光合作用的对比代谢如何在细胞内整合。这项研究将涉及基于实验室的实验模式甲藻和金藻文化。使用显微镜,生理学,蛋白质组学和代谢组学的方法,这项工作将测试有关混合营养的最终原因和后果的假设。主要目的是确定1)诱导混合营养的环境控制,2)猎物质量对捕食者选择的作用,3)混合营养的细胞和分子控制,以及4)营养同化和综合代谢。使用这些不同的研究方法,这项工作将产生一个全面的看法几个mixotrophs和提供新的见解细胞,生态和进化方面的mixotrophs。本研究的结果将加深我们对海洋浮游植物兼养的生理和生态作用的理解,并为在实验室和现场研究这一过程提供急需的分子标记。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Inducible Mixotrophy in the Dinoflagellate Prorocentrum minimum
- DOI:10.1111/jeu.12198
- 发表时间:2015-07-01
- 期刊:
- 影响因子:2.2
- 作者:Johnson, Matthew D.
- 通讯作者:Johnson, Matthew D.
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Matthew Johnson其他文献
Developing Socially Responsible Leadership and Social Perspective-Taking in Fraternities and Sororities: Findings From a National Study
培养兄弟会和姐妹会的社会责任领导力和社会视角:一项全国研究的结果
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
Matthew Johnson;Erica L. Johnson;John P. Dugan - 通讯作者:
John P. Dugan
Deposited in DRO : 09 November 2015 Version of attached
存于 DRO : 2015 年 11 月 9 日 附件版本
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
Carl Feghali;Matthew Johnson;D. Paulusma - 通讯作者:
D. Paulusma
コンピュータグラフィックスによる降雪表現手法の開発
利用计算机图形学开发降雪表达方法
- DOI:
- 发表时间:
2008 - 期刊:
- 影响因子:0
- 作者:
竹田恭平;水上嘉樹;玉木徹;金田和文;多田村克己;Matthew Johnson;竹田恭平;木下 理恵 - 通讯作者:
木下 理恵
CGによるガラス面上を流れる水滴表現手法の開発
开发使用 CG 来表现玻璃表面上流动的水滴的方法
- DOI:
- 发表时间:
2007 - 期刊:
- 影响因子:0
- 作者:
Matthew Johnson;玉木徹;金田和文;水上嘉樹;多田村克己;木下 理恵;島田 洋輔;竹中 秀一 - 通讯作者:
竹中 秀一
Recent Work Title Temperature-dependent quantum efficiency degradation of KCs-Sb bialkali antimonide photocathodes grown by a triple-element codeposition method
近期工作标题 三元共沉积法生长的 KCs-Sb 双碱锑化物光电阴极的温度依赖性量子效率退化
- DOI:
- 发表时间:
2017 - 期刊:
- 影响因子:0
- 作者:
Z. Ding;S. Karkare;Jun Feng;D. Filippetto;Matthew Johnson;S. Virostek;F. Sannibale;J. Nasiatka;M. Gaowei;J. Sinsheimer;E. Muller;J. Smedley;H. Padmore - 通讯作者:
H. Padmore
Matthew Johnson的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Matthew Johnson', 18)}}的其他基金
SBIR Phase I: Scalable Magnetically-Geared Modular Space Manipulator for In-space Manufacturing and Active Debris Remediation Missions
SBIR 第一阶段:用于太空制造和主动碎片修复任务的可扩展磁力齿轮模块化空间操纵器
- 批准号:
2335583 - 财政年份:2024
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
Collaborative Research: Evolution of acquired phototrophy by organelle sequestration in Mesodinium ciliates
合作研究:中纤毛虫通过细胞器隔离获得的光养进化
- 批准号:
2344640 - 财政年份:2024
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
Collaborative Research: Quantifying the impact of oxylipin chemical signaling on microbial community dynamics and biogeochemical cycling
合作研究:量化氧脂素化学信号对微生物群落动态和生物地球化学循环的影响
- 批准号:
2231922 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Continuing Grant
Elucidating the transient nature of electron transfer complexes at the single-molecule level
阐明单分子水平上电子转移复合物的瞬态性质
- 批准号:
BB/V006630/1 - 财政年份:2021
- 资助金额:
$ 61.85万 - 项目类别:
Research Grant
Research: Practices of Engineers in Rural Schools Involving Students and Teachers (PERSIST) in Engineering
研究:乡村学校工程师参与学生和教师的实践(PERSIST)
- 批准号:
1930777 - 财政年份:2019
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
Collaborative Research: Diversity of Physcomitrium pyriforme in North America and Europe: significance of autopolyploidy within a phylogenomic and experimental framework
合作研究:北美和欧洲梨形小须藻的多样性:系统发育和实验框架内同源多倍体的重要性
- 批准号:
1753800 - 财政年份:2018
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
Quantification of the forces that mediate electron transfers between proteins
介导蛋白质之间电子转移的力的量化
- 批准号:
BB/P002005/1 - 财政年份:2017
- 资助金额:
$ 61.85万 - 项目类别:
Research Grant
Collaborative: RUI: IRES: Birds, Beans, and Bugs; Modeling a Warming Climate's Effect on the Natural Enemies Hypothesis
协作:RUI:IRES:鸟类、豆子和虫子;
- 批准号:
1657973 - 财政年份:2017
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
Doctoral Dissertation Improvement Award: The Role of Heritage in Community Organization
博士论文改进奖:遗产在社区组织中的作用
- 批准号:
1630141 - 财政年份:2016
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
REU Site: Natural Resource Science on Native American Lands
REU 网站:美洲原住民土地上的自然资源科学
- 批准号:
1559943 - 财政年份:2016
- 资助金额:
$ 61.85万 - 项目类别:
Standard Grant
相似国自然基金
生理/病理应激差异化调控肝再生的“蓝斑—中缝”神经环路机制
- 批准号:82371517
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
羊草子株出生、发育及成穗的生理与分子机制
- 批准号:31172259
- 批准年份:2011
- 资助金额:56.0 万元
- 项目类别:面上项目
相似海外基金
An Integrated Model of Contextual Safety, Social Safety, and Social Vigilance as Psychosocial Contributors to Cardiovascular Disease
情境安全、社会安全和社会警惕作为心血管疾病社会心理因素的综合模型
- 批准号:
10749134 - 财政年份:2024
- 资助金额:
$ 61.85万 - 项目类别:
Elucidating the physiological and ecological factors that cause diversification of food-derived toxins in the Japanese natricine snake, Rhabdophis tigrinus
阐明导致日本水蛇(Rhabdophis tigrinus)食物源性毒素多样化的生理和生态因素
- 批准号:
22KJ1886 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Identifying the Effects of Race-Related Stressors on Laboratory- Induced Stress and Craving among African Americans with Alcohol Use Disorder
确定种族相关压力源对患有酒精使用障碍的非裔美国人实验室诱发的压力和渴望的影响
- 批准号:
10664454 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Evaluating Microaggressions among Latinx Individuals with Obesity
评估拉丁裔肥胖人群的微攻击行为
- 批准号:
10725858 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Detecting Adolescent Suicidality Biometric Signals and Dynamic Variability with Wearable Technology
利用可穿戴技术检测青少年自杀生物特征信号和动态变异性
- 批准号:
10731651 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Development of a digital therapeutic targeting anxiety sensitivity to reduce PTSD-SUD in women presenting for emergency care after sexual assault.
开发一种针对焦虑敏感性的数字疗法,以减少性侵犯后寻求紧急护理的女性的 PTSD-SUD。
- 批准号:
10449766 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Cyclical deficits in emotion regulation as a risk factor for alcohol misuse in premenopausal females
情绪调节的周期性缺陷是绝经前女性酗酒的危险因素
- 批准号:
10581399 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Investigation of Sex and Gender Differences in Cardiovascular Risk in Rural Communities
农村社区心血管风险的性别和性别差异调查
- 批准号:
10608716 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Adaptation to an anthropogenic habitat: Integrated modelling of ecological and physiological impacts on chacma baboons
适应人类栖息地:对查克玛狒狒的生态和生理影响的综合建模
- 批准号:
2870261 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别:
Studentship
Cranial electrotherapy stimulation: Piloting a road to PTSD prevention in first responders
颅脑电疗刺激:在急救人员中试行预防 PTSD 的道路
- 批准号:
10853457 - 财政年份:2023
- 资助金额:
$ 61.85万 - 项目类别: