Probing Mechanical Biomarkers with Microacoustofluidics: A Fluid-Structure Interaction Approach

用微声流控探测机械生物标志物：流固相互作用方法

• 批准号: 1438126
• 负责人: Francesco Costanzo
• 金额: $ 30万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-10-01 至 2018-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1438126&HistoricalAwards=false
• 关键词: Probing; Mechanical; Biomarkers; Microacoustofluidics; Fluid

PI: Costanzo, FrancescoProposal Number: 1438126The objective of the proposed work is to study the hydrodynamic interactions between deformable microparticles, and specifically between a micro-bubble and a cell. The idea is to create a micro-bubble using a laser next to the cell, and then use acoustic methods to obtain information about the mechanical properties of the cell. Such information would be used mainly for diagnostic purposes, but also for therapeutic purposes. This idea that a cell's mechanical properties can be used as a biomarker for pathogenic processes is currently being used to diagnose malaria, and there is some evidence that mechanical biomarkers may be used to diagnose cancer. The proposed work could lead directly from fluid dynamics research to applications. The proposed research will lead to more effective, cheaper, and faster cell-based on-chip diagnostic and therapeutic devices. As such, this research can have a major impact on public health world-wide.Cellular mechanical properties have been found to be valuable indicators for pathogenesis and pathophysiology. This has led to the identification of a new class of biomarkers: mechanical biomarkers that offer some advantages over traditional biochemical biomarkers. While a number of mechanical biomarker-based microfluidic devices have already been proposed in the literature, the full potential of mechanical biomarkers in microfluidic-based diagnostics and therapeutics has yet to be revealed. One reason is the fact that no techniques are currently available for the quantitative assessment of cell deformability in relation to the forces acting on them. Current approaches for estimating the radiation forces on objects in streaming flows are based on classical solutions for idealized geometries (typically spheres) and small deformation of elastic inclusions in the flow. The proposed research will use computational techniques based on the immersed finite element method to advance knowledge in these areas. The goal is to relate cell deformability to the hydrodynamic forces imposed on a cell or on a group of cells in a microfluidic device. The validation of the proposed computational framework will be done against experiments with cancer cells in an opto-thermally-generated and acoustically-activated surface bubbles microfluidic device. The co-PIs propose to involve undergraduate students in research and to leverage already existing initiatives at Penn State in order to reach underrepresented minority students: the Women in Engineering Program and the Multicultural Engineering Program.This award by the Fluid Dynamics Program of the CBET Division is co-funded by the Instrument Development for Biological Research (IDBR) Program of the Division of Biological Infrastructure.

主要研究者：Costanzo，Francesco提案编号：1438126拟议工作的目的是研究可变形微粒之间的流体动力学相互作用，特别是微泡和细胞之间的相互作用。 这个想法是使用激光在细胞旁边产生一个微气泡，然后使用声学方法获得有关细胞机械特性的信息。 这些信息将主要用于诊断目的，但也可用于治疗目的。 细胞的机械特性可以作为致病过程的生物标志物，这一想法目前正被用于诊断疟疾，并且有一些证据表明机械生物标志物可以用于诊断癌症。所提出的工作可以直接从流体动力学研究到应用。这项研究将导致更有效，更便宜，更快的基于细胞的芯片诊断和治疗设备。因此，这项研究可能对世界范围内的公共卫生产生重大影响。细胞力学特性已被发现是致病机理和病理生理学的有价值的指标。这导致了一类新的生物标志物的识别：机械生物标志物，提供了一些优于传统的生物化学生物标志物。虽然文献中已经提出了许多基于机械生物标志物的微流体装置，但机械生物标志物在基于微流体的诊断和治疗中的全部潜力尚未被揭示。一个原因是，目前没有技术可用于定量评估细胞变形性与作用于它们的力的关系。目前的方法来估计物体上的辐射力在流的流动是基于经典的解决方案，理想化的几何形状（通常是球体）和小变形的弹性夹杂物的流动。拟议的研究将使用基于浸没有限元法的计算技术来推进这些领域的知识。目标是将细胞变形性与施加在微流体装置中的细胞或细胞组上的流体动力相关联。所提出的计算框架的验证将针对癌细胞在光热产生和声激活的表面气泡微流体装置中的实验进行。共同PI建议让本科生参与研究，并利用宾夕法尼亚州立大学现有的举措，以达到代表性不足的少数民族学生：妇女在工程计划和多元文化工程计划。这个奖项由CBET部门的流体动力学计划共同资助，由生物基础设施部门的生物研究仪器开发（IDBR）计划资助。